autoPulse® - TFF System for High-Throughput Sample Concentration and Diafiltration
Key Features:
- High-Throughput
Process up to 54 samples per run with up to 4 processed in parallel. - Low Hold-Up Volume
Final volume as low as 250 µL with ±25 µL resolution. - High-Flux Performance
Dual-diaphragm pumps enable permeate flow up to 1.7× faster than µPulse and 4× faster than centrifugal filters. - Buffer Flexibility
Use up to 4 external buffer reservoirs or on-deck conical tubes. - Automatic Cleaning-in-Place
Automatically cleans the liquid path and performs buffer/water rinsing for optimal sample conditions. - Disposable Fluid Path
Re-engineered disposable filter chips with integrated transfer tubing eliminate cross-contamination. - Remote Monitoring
Intuitive web-based software allows full protocol setup, remote monitoring, and run log exporting.
Run Versatile Samples:
- Nanoparticle Processing
Efficiently process lipid nanoparticles, liposomes, and polymeric nanoparticles for drug delivery and therapeutic efficacy. - Protein Processing
Optimize protein preparation with fast and gentle processes including concentration, formulation, desalting, and refolding. - Bioconjugate Cleanup
Ensure gentle and efficient removal of small unconjugated molecules from crude biomolecular labeling reactions. - Nucleic Acid Processing
Simplify in vitro synthesis of RNA and linear or plasmid DNA with efficient concentration and buffer exchange. - Cell & Particle Harvesting
Streamline the harvesting of cells, extracellular vesicles, enzymes, and Virus-Like Particles (VLPs) with high yield and quality. - Virus Processing
Gently concentrate and exchange buffers for AAVs, bacteriophages, and lentiviruses while preserving structural integrity. - Vaccine Development
Optimize DNA, RNA, and polysaccharide vaccine formulations for stable, cost-efficient results.
Membrane Specifications
Available membrane materials and MWCOs sizes: mPES: 5, 10, 30, 50, 100 and 300 kDA
RC: 5, 10, 30 and 100 kDA
Membrane area: 3.5-12 cm2
Membrane Storage: 40% Glycerol, 0.004% Thiomersal
System Specifications:
Recommended Starting Volume: 1 mL - 100 mL
Minimum Final Volume: 250 µL
Suggested Max. Sample Viscosity: 100 cP
SBS format labware
Buffer Reservoirs: 50mL conicals on deck or external buffer reservoirs
External permeate collection with overflow monitoring
Data management meets 21 CFR Part 11 standards for GMP
Electrical Specifications:
110 V - 240 V, 50 Hz - 60 Hz, 50 W typical, 150 W max. All outlet types are supported
Connectivity: WiFi/Ethernet
Dimensions:
Height: 830 mm (32.7”)
Height: 1070 mm (42.2”) (with door open)
Width: 835 mm (32.9”)
Depth: 740 mm (29.1”)
Weight: 81 kg (178.6 lbs)