- Home
- Companies
- Thermo Fisher Scientific, LIMS & ...
- Products
- Thermo Scientific - Selectris and ...
Thermo Scientific - Selectris and Selectris X Imaging Filters
Cryo electron microscopy imaging enhanced with zero-loss energy filtering for atomic-resolution cryo-EM. Available on Thermo Scientific Krios and Glacios Cryo-TEMs, the Thermo Scientific Selectris and Selectris X Imaging Filters are post-column imaging filters that improve the contrast of TEM images, resulting in high-resolution structures up to atomic resolution. The zero-loss filtering of the Selectris Filters removes noise caused by inelastically scattered electrons, producing an increased signal-to-noise ratio (SNR) and better contrast. Designed for high stability, the zero-loss peak position of the Selectris Filters is insensitive to temperature variations in the environment, eliminating the need for frequent tuning.
With the Selectris Filters, zero-loss energy filtering is straightforward due to the thorough integration of software and hardware along with extensive automation and exceptional stability. Every mechanical and electron-optical element has been designed for stability and data reproducibility, enabling the unattended and reliable acquisition of large datasets with narrow energy slit widths (less than 10 eV). The capability to use these narrow slit widths provides an additional boost in contrast for single particle cryo-electron microscopy (cryo-EM), enhancing signal strength, resolution, and throughput.
Paired with the latest generation Thermo Scientific Falcon 4 Direct Electron Detector, Selectris Filters allow you to obtain high-resolution structures quickly, revealing new biological insights. To further increase the productivity and ease-of-use of your cryo-EM workflows for single particle analysis and cryo-electron tomography, these filters are fully integrated into Thermo Scientific EPU Software, Thermo Scientific Tomography Software, and our instrument operation software.
On the rare occasion that filter tuning is necessary, it can be completed automatically within minutes; this ensures smooth daily operation and efficient data acquisition.
Selectris X Filter – taking the next step toward atomic resolution
Expanding on the stability, ease-of-use, and performance of the Selectris platform, the Selectris X Imaging Filter offers an even more sophisticated electron optical system for further aberration correction. This results in extremely low distortion characteristics in both the image and energy domains, opening the way to true atomic-resolution structures.
- Narrow (<10 eV) zero-loss energy filtering offers enhanced contrast for higher resolution reconstructions
- Sophisticated aberration correction, low image distortions, and uniform energy resolution over entire field of view
- Minimized sensitivity to temperature variations
- Fully integrated with Thermo Scientific instrument operation software, as well as EPU Software and Tomography Software for data collection.
- Automated filter tuning completed in minutes
- No need to interrupt data collection for zero-loss centering
- High detective quantum efficiency (DQE)
- High speed, routinely capable of producing 300+ movies/hour
- Built-in electron event representation (EER) for full temporal resolution (240 fps to disk) - no need for fractionating at time of acquisition
- Super resolution without file size penalty
Imaging Filter
- Position: Bottom mounted
- Stability: Slit drift < 1eV/K
- Zero-loss centering: Automated
- Filter tuning: Automated
- TEM operating voltage: 200 kV, 300 kV
Direct Electron Detector Camera
- Sensor: 4,096 × 4,096 pixels, ~ 5.7 x 5.7 cm
- Pixel size: 14 x 14 um2 pixel size
- File formats:
- EER super-resolution up to 16k x 16k. All counted frames (~240 fps) accessible/written to disk, fractionation not required
- MRC
- Detection modes:
- Electron counting mode
- Survey mode (fast linear mode)
- Automation:
- Integrated in Thermo Scientific EPU and Tomography software
- Compatible with 3rd party software via TEMscripting
