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Thomson - Model BIOAdvantage -High Performance Columns and Guard Systems
C18, C8 & C4 (100, 200 & 300 Angstrom) for peptide, protein and biomolecule analysis. Unique Base Deactivated columns. Exceptional separations for amines and polar molecules. 300Å or 200Å for Peptides and Proteins. Available in stainless steel or PEEK. Designed to use the Direct Connect Guard System. Thomson reverse phase columns are designed for the chromatography of peptides and proteins. They are characterized by high resolution, efficiency and peak symmetry.
Available in C4, C8 or C18 phases, 100Å, 200Å, or 300Å pore sizes and 3µm or 5µm particle sizes.
Take advantage of the better resolution, for small molecules, afforded by the high surface area, 100Å packing, or separate larger molecules on the 300Å packing. Their identical selectivity allows you to maintain the same elution order from one product to the other.
Chromatographing small proteins or peptides on a 300Å packing is conventional wisdom. But in many cases the higher surface area support would be the better choice. Now we offer an intermediate surface area with our 200Å materials.
These columns, perfect for LC/MS, provide excellent resolution without TFA or other modifiers. Or chose a modifier other than TFA, to enhance selectivity for the compounds of interest.
BIOAdvantage Basic & BHS are unique materials ideally suited for chromatographing basic compounds. Compare the chromatogram of Pyridine & Phenol in 40% acetronitrile/water unmodified by TFA or other enhancing additive.
Other columns, thought to be the standard of the industry, make these compounds tail badly and require suitable modifiers to “shield” active sites on the solute or packing surface. BIOAdvantage provides distinctive peaks without additives.
- Hodges Peptide Mix
- Tryptic Digest of Transferrin
- Parabens
- Synthetic Oligonucleotide
High performance analytical columns and cartridges require a protection device equally as efficient.
Direct Connect Guards are a highly efficient column protection device that guards the analytical column from both particulate matter and chemical contamination.
Its new, unique PEEK holder is precision manufactured to very tight tolerances and can be hand tightened to 5000 psi.
Direct connection to your HPLC column results in zero dead volume.
The holder is supplied with a tube stop depth compatible with most of today’s HPLC fittings. If you need a different stop depth, it’s a simple matter to change the connecting tube to accommodate your HPLC column’s end fitting.
The small volumes and direct connection means performance loss due to our guard is minimal if even measurable.
They will not alter the selectivity of your analytical column.
- Temperature: 80°C maximum
- Pressure: ~ 5000 psi
- Variance: Approximately 20µl2 at 200µl/min. This means, under isocratic conditions, some performance loss will be experienced with column ID’s less than 4.6 mm.
- Solvents: Compatible with most solvents. DMSO, Me2Cl2 and THF cause PEEK to swell over time. So they are not recommended for routine use.
BioAdvantage HL, High Load, is intended for separations requiring maximum loading and selectivity. The high carbon load, 18%, and absence of micro-pores eliminates the problems of other high capacity columns.
- Column: BioAdvantage HL C18, 5µm, 4.6 x 150mm
- Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Acetonitrile
- Gradient: Linear gradient, 1 ml/min, 0 to 30% solvent B in 30 minutes.
- Detection: UV 210 nm
- Discussion: The Canadian corporation, SPI, produces a well characterized, challenging peptide mixture that is very useful for quantitying HPLC column performance for peptides. The difficult to separate first pair of decapeptides are near base line resolved on Advantage HL C18 columns.
Peak Identities:
- H2N-RGAGGLGLGK-amide
- AC-RGGGGLGLGK-amide
- AC-RGAGGLGLGK-amide
- AC-RGVGGLGLGK-amide
- AC-RGWGLGLGK-amide
BioAdvantage 100, a work horse, for years has been the choice for separations of small molecules like carbamate pesticides, pharmaceuticals, synthetic oligonucleotides and small peptides. It is characterized by high efficiency symmetrical peaks and long life
Column: BioAdvantage 100, C18, 5µm, 4.6 x 250mm
Mobile Phase: A: 50 mM phosphoric acid, pH 2.8
B: 60% Acetonitrile / 40% A
Gradient: 1 mi/min
0 min 0%B
5 min 0%B
120 min 40%B
130 min 60%B
Detection: UV 215 nm
Instrument: PerSeptive Biosystems Integral
Column: BioAdvantage HL, C18, 5µm, 4.6 x 100mm
Mobile Phase: A: 70% Acetonitrile in water
Detection: UV 254 nm
Discussion: Fast analysis, symmetrical peaks and baseline resolution are characteristics of paraben analysis on short, high carbon load C18 columns.
Peak Identities:
- Methyl paraben
- Ethyl paraben
- Propyl paraben
- Butyl paraben
Column: BioAdvantage 100, C18, 5µm, 4.6 x 100mm
Mobile Phase: A: 0.1M TEAA, pH 7.0
B: Acetronitrile
Gradient: Linear, 1ml/min from 8-15% ub 24 mins.
Detection: UV 260 nm
Discussion: Trityl-off chromatography is a routine method used for purification and purity determination of synthetic oligonucleotides.
