YMC Contichrom - Model HPLC -® TWIN for Peptides & Oligonucleotides
Conventional purification of peptide and oligonucleotides is a trade-off between yield and purity. YMC Contichrom Twin two-column configuration facilitates continuous chromatography for ‘tides purification with automatic internal re-cycling and re-separation of overlapping fractions until maximum yield and purity are achieved. This GMP-ready, easy-to-use chromatography system features the exclusive MCSGP technology developed? by YMC ChromaCon AG. Enhance yield by up to 3-fold while maintaining high purity and enjoying up to 70% reduction in solvent and buffer consumption.
- Reduction in solvents (buffers) by up to 70%
- Improved yield 30-60%
- Process faster, significant increase in productivity
- Simple design to facilitate validation
- Industry-leading high-pressure pumps for maximum flow accuracy and precision throughout a wide gradient range.
- Scale-up/-down from Contichrom® HPLC bench-top instrument
- The YMC EcoPrime Twin HPLC is capable to operate not only the MCSGP but also batch mode. This single system solution allows for ultimate flexibility for manufacturing saving space and capital equipment cost without investing in additional (batch) equipment.
Peptides are a highly versatile class of drugs that cover a wide variety of pharmaceutical targets, have high specificity and are generally low in toxicity. A major challenge, however, is that their purification can be difficult, due to weakly or strongly retained impurities overlapping with the product peak. This results in poor yield at the desired purity requiring larger or more numerous batches to generate sufficient quantities.
Conventional chromatographic processes can produce fractions with the desired target purity but often at the expense of yield while at the same time consuming significant quantities of solvents and buffers. The opportunity is to employ an innovative, automated continuous chromatographic approach which eliminates the dilemma of choosing between yield for purity and also provides significant savings in solvent and buffer consumption. The reduction in synthesis batches required with YMC’s higher yield purification system is a direct outcome that further enhances ROI.
Single-stranded DNA and RNA oligonucleotides are increasingly being used in a range of areas from clinical diagnostics to new biotherapeutics. While the automated synthesis of oligonucleotides is a rather straight-forward process, purification still presents challenges. Efficient purification of target oligonucleotides depends on their length and often yield is sacrificed to obtain a higher purity fraction. The MCSGP process performed with Contichrom TWIN HPLC enables re-cycling and reseparation of fractions with product purity below the threshold to obtain a high purity fraction at higher yield.
The Contichrom TWIN HPLC is based on YMC ChromaCon’s patented approach to continuous purification. The simple, two-column design is built for use in GMP environments where yield, purity, profitability, and up-time are paramount. It is well suited to transform existing batch processes using your choice of chromatography resin and columns.