Twist - Precision Combinatorial Variant Library Technology

Combined with our well established molecular biology expertise, the Twist oligo synthesis platform enables the fabrication of highly diverse gene mutant libraries with excellent variant representation and highly specific user-defined composition with no unwanted bias or motifs. Twist library technology enables a comprehensive interrogation of the variant sequence space.

High Diversity Precision

• Effortlessly incorporate multiple variant domains into one or multiple scaffolds
• Precise control over codon usage (all 64 codons), amino acid distribution, and length variation

Verified Quality

• Rigorous quality control, including NGS verification of modified regions
• Sequence variant ratios documented
• Uniform distribution of user-defined variants
• No premature stop codons or unwanted codons

Flexibility

• Design all sequences, single or multiple domains and combinations— single, pairwise, or triple variants
• Modular synthesis system enables iteration of future libraries
• Greater flexibility than TRIM and the degenerate approach