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Baltic Biomaterials Centre of Excellence
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  5. Waters - Model ACQUIT Y UPLC - Ultra ...

Waters - Model ACQUIT Y UPLC -Ultra High Performance Liquid Chromatograph Equipped with UV, Fluorescence and ELS Detectors

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The Waters® ACQUIT Y UPLC® H-Class System delivers the flexibility of quaternary solvent blending with the advanced performance of UPLC® separations. The system’s holistic design is targeted for routine analysis and method development use and is perfectly suited for running both HPLC and UPLC applications while still realizing the improved resolution and sensitivity of UPLC separations. The system is comprised of a Quaternary Solvent Manager (QSM), a Sample Manager with Flow-Through Needle (SM-FTN) design, and offers a choice of column compartment products.

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  • ACQUITY UPLC H-Class System Instrument Specifications
  • Flow precision  0.075% RSD or ±0.020 min SD, whichever is greater, based on six replicates (with i2 Valve) 60:40 H2O/MeOH via Auto•Blend Plus Technology, 0.5 mL/min, alkylphenone mix (5.0 μL injection volume), ACQUIT Y UPLC BEH C18, 1.7 μm, 2.1 x 50 mm, 35 °C ± 0.3 °C, UV @ 254 nm
  • Composition ripple (baseline noise)  i2 Valve)  
  • A: H2O + 0.1% TFA, B: ACN + 0.1% TFA, 0.5 mL/min, ACQUIT Y UPLC BEH C18, 1.7 μm, 2.1 x 50 mm UV @ 214 nm, 10 mm analytical flow cell
  • Composition accuracy  ±0.5% absolute (full scale) from 5% to 90% from 0.5 to 2.0 mL/min (with i2 Valve) 
  • Degassed ACN/H2O (90:10), ACN/H2O (90:10) with caffeine at 12 mg/L concentration, back pressure 2000 psi, step gradient method, UV at 273 nm
  • Composition precision  i2 Valve) 
  • 60:40 H2O/MeOH via Auto•Blend Plus Technology, 0.5 mL/min, alkylphenone mix (5.0 μL injection volume), ACQUIT Y UPLC BEH C18, 1.7 μm, 2.1 x 50 mm, 35 °C ± 0.3 °C, UV @ 254 nm 
  • Compressibility compensation   Automatic and continuous 
  • Priming  Wet priming can run at flow rates up to 4 mL/min
  • Pump seal wash  Equipped with an automated wash system to flush the rear of the high pressure seal and the plunger
  • Flow ramping  Range: 0.01 to 30.00 min to reach 2.0 mL/min 
  • Default: 0.45 min to reach 2.0 mL/min