Nippon FastGene® - Model FG-CH01 -Western ECL Kit – Chemiluminescent Western Blot Detection
The FastGene® Western ECL Kit is an enhanced chemiluminescent (ECL) substrate based on luminol. It is used to detect horseradish peroxidase (HRP) – conjugated secondary antibodies. The high femtogram or low picogram detection of antigen is enabled by FastGene® Western ECL Kit brilliant sensitivity and long signal duration. This long chemiluminescent signal duration makes it possible to detect signals on both digital and film-based imaging systems without any loss in signal intensity. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.
- Chemiluminescent substrate based on luminol
- High sensitivity – Detection of antigens with very low concentrations
- Long signal duration – Optimal for digital and film-based imaging
- Fast protocol – 10-30 sec incubation time
- No optimization required – Easy switch from other brands
- Perfect for PDVF and nitrocellulose membranes
- Compatible with Western Blotting Markers
Mix the luminol solution and peroxide solution in a 1:1 ratio, and thoroughly agitate the chemiluminescent substrate solution well for preparing the 0.1 ml of solution / cm2 of membrane.
Place the membrane with the protein side up and remove the membrane from the chemiluminescent substrate solution. Image the membrane with a digital imager.
Comparison between the FastGene® Western ECL Kit and competitor products
A
1000-100 ng BSA were separated in a 4-20% SDS-Page (FastGene® Precast Gel) using a primary antibody 1:5000 alpha-BSA (Invitrogen, A11133, corresponding to 4 µg). All ECL kits were used under the same experimental workflow.
B
An IL-6 fusion protein was detected using a primary antibody against IL-6 from mouse and a secondary anti-mouse peroxide (POD) antibody 2 seconds exposure for the Western Blot. All ECL kits were used under the same experimental workflow.
