InVivo BioTech Services GmbH a BRUKER Company
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Antibody Sequencing And Optimization Services
If you have a hybridoma cell line but your antibody yield is low, or if your antibody is specific but needs to be humanized, then InVivo can help you overcome these obstacles. By sequencing the cDNA of the variable domains from heavy and light chain, you will be able to optimize the antibody according to your needs. From RNA extraction to sequencing, from codon optimization to high yield antibody production and purification – get it all from one source!
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Antibody Sequencing
- Total RNA isolation from hybridoma cell line
- cDNA synthesis (via either oligo dT or species-specific primers)
- Amplification of variable domains of heavy and light chain
- Subcloning into sequencing vector and sequencing
Antibody Optimization
- Codon optimization
- Antibody engineering
- Subclass switches to human (chimeric antibodies)
Related Services
- Transient antibody expression in HEK-INV or CHO cells in serum-free medium
- Antibody purification by protein-A/-G affinity chromatography
