Determination of Amino Acid Composition
Creative Proteomics can help you to determine the amino acid composition of various samples you submit. As the basic brick for peptides and proteins ,the amino acids are quite important because of the significance of protein/peptide physiological functions. Meanwhile the amino acid composition and levels are essential for nutrition and status of body health. For amino acid metabolomics, there are more than 300 kinds of amino acids and ~20 amino acids can be founded in natural proteins.
Currently there are 3 ways for amino acid analysis: HPLC, automatic amino acid analyzer and LC-MS/MS. In the most experiments, the analytical procedures usually include hydrolysis of protein/peptides in the samples, derivatization of free amino acids pre/post column, separation and quantitation of the amino acids. Not only the 20 natural amino acids, but also all the known amino acids which may exist in the biological samples, such as γ－aminobutyric acid (GABA), L-Ornithine, can be identified and quantified with proper internal standards and calibration curves.