DNA, RNA and Protein Analysis
DNA and RNA purification and analysis services include isolation of genomic DNA, mRNA, small DNA and RNA sequences from cells or tissues and analysis by quantitative real time PCR (QRT-PCR). SIA has vast experience in isolation and analysis of RNA and DNA sequences by PCR and QRT-PCR.
Gene expression analysis services include all steps form mRNA isolation through cDNA preparation and quantitative real time PCR analysis (QRT-PCR).
Protein Analysis by Western Blotting and Immunofluorescence is used to assess the specific proteins of interest, their amounts, stability, posttranslational modifications and localization within cells. Co-staining of two cellular proteins or cellular protein and a molecule of interest, may reveal co-localization and possible interaction.
Immunohistochemistry (IHC) services include immunolocaliztion of either one or several antigens of interest in frozen, paraffin-embedded tissue sections and cell samples. We utilize various detection methods such as immunofluorescence, alkaline phosphatase, and immunoperoxidase.