LUMI-CELL - For Estrogen Receptor - α (ERα)

Traditional instrumental analysis methods can provide outstanding high-resolution quantitative results for the known estrogenic chemicals that are being measured. However, the major limitations of instrumental analysis methods for estrogenic chemical screening and monitoring purposes is that they are not able detect unknown estrogenic chemicals and they can’t provide a measure of the total estrogenic activity of a sample extract. In contrast, ER-based cell bioassays provide an avenue in which to detect all estrogenic chemicals in a sample extract (known and unknown) and determine its total estrogenic activity. These aspects, combined with the fact that cell bioassays are also significantly less time-consuming and costly as instrumental analysis make them the preferred method of choice for open screening and monitoring purposes.

This figure shows the results of analysis of the estrogenic activity of selected chemicals. While the estrogenic potency and efficacy of the synthetic estrogen, Diethylstilbestrol (DES), was similar to that of 17β-estradiol (E2), numerous phyoestrogens (Genistein (from soy), Daidzein, and Zearalenone (mycotoxin)) and other compounds showed reduced potency but were efficacious ER activators. These results demonstrate the utility of the LUMI-CELL^® bioassays for detection and relative quantitation of estrogenic chemicals.