MtoZ Biolabs - Model glycosylated-analysis -Glycan Profile Analysis Service
For the relative quantification of glycan types in glycosylated proteins, MtoZ Biolabs provides glycan type relative quantification services based on MALDI-TOF/LC-MS. Due to the diversity of O-glycosylation modifications and the absence of common enzymes that can cleave all O-glycans, our glycan type analysis currently focuses only on N-glycosylated proteins.
The strategy for glycan type analysis is as follows: all N-glycans in the target protein are digested by PNGase F enzyme, followed by labeling N-glycans with 2-AA, and specifically enriching N-glycans through an SPE column. Depending on the complex glycosylation of the target protein, MALDI-TOF-TOF or LC-MS/MS is used to detect 2-AA-labeled N-glycans, and GlycoWorkbench software (CFG database) is used to identify N-glycans and analyze the relative abundance of glycan types based on the mass spectrometry peak areas of the glycans.
1. Relatively Short Identification and Analysis Time
2. High Sensitivity and Good Reproducibility
1. Identification of N-glycan Types in Glycosylated Proteins
2. Analysis of the Relative Abundance of N-glycan Types in Glycosylated Proteins
1. If you provide tissue samples, please transport them on dry ice.
2. If you provide protein samples, please transport protein extracted from ordinary tissue and cell lysis buffers.
3. Sample Transport: Please use an ample amount of dry ice and opt for the fastest possible mailing method to minimize sample degradation during transportation.
1. Experimental Procedures
2. Mass Spectrometric Parameters
3. Protein N-glycan Types and Relative Abundance
4. Mass Spectrometric Images
5. Raw Data
1. N-glycan Analysis Service
2. O-glycan Analysis Service
MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.
Email: marketing@mtoz-biolabs.com