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MtoZ Biolabs - Model pharm-glycosylation-site-analysis -Glycosylation Site Detection Service
Glycosylation, especially N-glycosylation, is a universal post-translational modification in regarding the localization, function, activity of proteins in tissues and cells. The study of the N-glycosylation site is an important prerequisite for understanding the function of sugar chain of the target protein. Among the information related to N-glycosylation, the site of N- glycosylation is the basis for our understanding of its sugar chain function. Besides the application of point mutation in traditional biochemistry, the study of glycosylation sites has also played an important role in the identification of glycosylation sites in N-terminal. MALDI TOF MS and nano LC-ESI-MS/MS are commonly used to identify the N/O-glycosylation sites in the antibodies. MtoZ Biolabs uses both MALDI TOF MS and nano LC-ESI-MS/MS technology to provide efficient and accurate glycosylation analysis of multiple biopharmaceuticals, including proteins, antibodies, etc. Upon the sample reception, Fc domain and Fab of the antibodies are first separated, followed by digestion into peptide fragments, and analysis of glycosylation site by MALDI TOF MS and nano LC-ESI-MS/MS.
Figure 1. Glycosylation Site Analysis of Antibody
1. Digestion of Biopharmaceutical Samples by Trypsin or Other Appropriate Proteases
2. Separation of N-Glycopeptides and O-Glycopeptides Through Chromatographic Column
3. Deglycosylation Treatment
4. Peptide Analysis by MALDI-TOF MS, or ESI-MS/MS
5. Analysis of the N/O-Glycosylation Site Based on the Mass Spectra Results
6. Comparison of the Analytical Results of Glycosylation Sites of Different Batch Samples
1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. MS Raw Data Files
4. Glycosylation Site Results
5. Bioinformatics Analysis
MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.
Email: marketing@mtoz-biolabs.com