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Ion AmpliSeq SARS-CoV-2 - Insight Research Assay
Unlock low-titer samples with superior sensitivity and robust performance. The Ion AmpliSeq SARS-CoV-2 Insight Research Assay leverages a unique targeted sequencing approach that yields improved coverage and uniformity from samples previously unobtainable by NGS. Incorporation of intelligent panel design provides an increased resilience to new mutations introduced within the viral genome. Integrated analysis tools enable automated consensus sequence generation, mutation annotation, and lineage assignment.
Genome coverage and uniformity vs Ct value using the Ion AmpliSeq SARS-CoV-2 Insight Research Assay
Demonstrated >98% Reads on Target and coverage uniformity obtained from sequencing samples with low viral titer as indicated by real-time PCR results. Sample measurements represent N=2 and average Ct value was used in analysis. Libraries were generated using the Ion AmpliSeq SARS-CoV-2 Insight Research Assay manual preparation protocol and sequenced on a Ion 540 Chip. Data courtesy of Paris Demo Lab using nasopharyngeal swab samples from Laboratoire Départemental d`Analyses du Jura - Lda39.
Ion Torrent targeted next-generation sequencing (NGS) technology enables a streamlined research workflow for complete viral genome sequencing and variant detection for epidemiological studies of SARS-CoV-2.
The Ion AmpliSeq SARS-CoV-2 Insight Research Assay consists of two pools with amplicons ranging from 125 bp to 275 bp in length and covers >99% of the SARS-CoV-2 genome, including all serotypes. The assay is part of a fast, automated, and accurate targeted NGS workflow that enables coronavirus typing in under a day. This end-to-end solution includes the assay and plug-in suite that was developed in collaboration with researchers at the forefront of the SARS-CoV-2 crisis. Our complementary targeted NGS systems makes SARS-COV-2 epidemiology research accessible to any lab, regardless of your team’s current level of NGS expertise.
Benefits of using Ion Torrent NGS for SARS-CoV-2 research:
- Rapid turnaround time
- Highly automated NGS workflow—for easier adoption, lower user intervention, and improved lab efficiency
- Accuracy of variants—lower substitution errors for SNVs
- Higher success rates—analyze samples directly with lower viral loads (as low as 20 copies)
The panel can be used for the following types of research applications:
- Viral identification and lifecycle
- Viral genome sequencing
- Viral surveillance and epidemiology research