- Home
- Companies
- MtoZ Biolabs
- Services
- MtoZ Biolabs - Edman Degradation Method ...
MtoZ Biolabs - Model n-terminal-sequence-analysis-using-edman-degradati -Edman Degradation Method for N-terminal Sequence Analysis Service
The N-terminal sequence is closely related to the function and stability of proteins. The analysis of the N-terminal sequence of proteins can determine the starting point of proteins, which is conducive to the analysis of the higher-order structure of proteins and reveals the biological functions of proteins. The Edman degradation method removes amino acids from the N-terminal of proteins step by step and identifies their types to determine the N-terminal amino acid sequence of proteins or peptides. The Edman sequencing reaction is a cyclical process. The peptide/protein reacts with phenyl isothiocyanate (PITC) under weakly basic conditions, and then is treated with acid. Only the amino-end residue is released from the peptide chain in the form of phenylthiohydantoin (PTH) derivative, and then the separated amino acid is identified. Each round of reaction can identify one amino acid, and in this way, the identification of the N-terminal amino acid sequence is finally achieved.
Figure 1. Schematic Diagram of N-terminal Sequence Analysis by Edman Degradation Method
MtoZ Biolabs has established an N-terminal sequence analysis platform based on the Edman sequencing system of Shimadzu Corporation, providing N-terminal sequencing services for purified protein products, antibodies, protein vaccines, recombinant collagens, etc., for researchers and research clients. Using our sequencing system, the sequence information of the N-terminal 30 amino acids all can be determined. With a specific protein sampling system, 60-70 N-terminal amino acids can be determined.Welcome to contact to learn more about our service!
Automatic Protein/Peptide Sequencer (PPSQ33A)
1. Direct Analysis of Amino Acid Information Through Sample Treatment via Gel Running or Dot Blotting, Enabling Accurate Analysis of the N-Terminal Amino Acid Sequence Without the Need for any Protein Database
2. Accurate Distinction among Amino Acids: Isoleucine, Leucine, Glutamine and Lysine
3. Accurate Identification and Determination of Truncated Forms (N-1, N-2, etc.) Mixed Within the Full-length Sequence
4. Accurate Measurement of Protein Sequences Containing Numerous Repeat Segments
A mixed standard of 19 PTH amino acids was tested to calibrate 19 types of PTH-amino acids with its calibration test chromatogram as follows:
Figure 2. Standard Calibration Test Chromatogram
After the calibration test of the PTH-amino acid mixed standard, the N-terminal sequence of the sample was tested. The sample test chromatogram is as follows:
1. Experimental Procedures
2. Mass Spectrometric Parameters
3. Detailed information on Edman degradation N-terminal sequence analysis
4. Mass Spectrometric Images
5. Raw Data
MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.
Email: marketing@mtoz-biolabs.com