- Home
- Companies
- MtoZ Biolabs
- Services
- MtoZ Biolabs - N/C Terminal Sequencing
MtoZ Biolabs - Model pharm-n-c-terminal-sequencing -N/C Terminal Sequencing
N- and C-terminal sequences are important structures and functional parts of protein and polypeptide, and they may play decisive roles in the biological function of protein. The main methods for protein C-terminal sequencing include carboxypeptidase, chemical and tandem mass spectrometry, and the common methods for N-terminal sequencing include Edman degradation sequencing and mass spectrometry. Each of these methods has the respective advantage and shortcoming. Therefore, the combination of a variety of different sequencing methods can adapt to the requirements of multiple protein sequencing. For example, Edman degradation may not well solve the problem of blockage and protein modification on the N-terminal end. This drawback can be overcomed by mass spectrometry analysis. MtoZ Biolabs uses both Edman degradation system and MALDI-TOF for N/C sequencing of biopharmaceutical products. This combination of two methods ensures accurate analysis of N-terminal blockage and modified protein N/C terminal.
Figure 1. Biopharmaceutical N/C-Terminal Sequencing
1. Sample Types: Lyophilized & Solution Samples
2. Amount of Total Protein: >300 ug
3. Purity: >90%
4. Content of Salt: Volatile Salt<200 mM; Non-Volatile Salt<5 mM
In this case, the N- and C-terminal sequence of one of our clients’ protein sample has been analyzed using a combination of Edman degradation and MALDI-TOF technologies. The sequencing data highlighted in red color accurately shows the N- and C-terminal sequence of the protein.
1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. Edman Sequencing and MS Raw Data Files
4. Biopharmaceutical N/C-Terminal Sequence Results
5. Bioinformatics Analysis
Identification of Biopharm
Variation Analysis
Antibody C-Terminal Variation Analysis
Purity Analysis
Host Cell Protein (HCP) Analysis
Antibody-Drug Conjugate (ADC) Analysis
MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.
Email: marketing@mtoz-biolabs.com