MtoZ Biolabs - Model n-glycosylation-site-occupation-analysis -N-Glycosylation Site Analysis Service | Biological Products

N-glycosylation is a post-translational modification (PTM) involved in various physiological and pathological processes. The variability of glycosylation modification site is a key indicator of cellular activity, as evidenced by the correlation between the reduction of glycosylation attachment site on serum protein and the severity of congenital disorders of glycosylation (CDGs). Therefore, accurately determining site occupancy rate is crucial for understanding the impact of protein glycosylation on human health.

To determine glycosylation attachment site, protein N-glycosidase F (PNGase F) is typically used in heavy water to separate polysaccharides from proteins. During this process, the originally glycosylated asparagine undergoes deamidation to become aspartic acid, with an addition of an Omodification. Because the introduction of O changes the molecular weight, glycosylation sites can be detected by comparing peptides containing aspartic acid with those containing unglycosylated asparagine. The general strategy for analyzing variable N-glycosylation site in glycoproteins includes protein digestion with PNGase F, polysaccharide release, separation of glycopeptide by liquid chromatography (LC), and detection by mass spectrometry (MS).

MtoZ Biolabs has much analytical service experience in glycomics and can customize glycomics analysis services according to your specific needs. Free consultation is available!

1. Experimental Procedures

2. Mass Spectrometric Parameters

3. Mass Spectrometric Images

4. Raw Data

5. Detailed Information on Identified Glycosylation Sites

MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.

MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.

Email: marketing@mtoz-biolabs.com