MtoZ Biolabs - Model n-terminal-sequencing -N-Terminal Sequencing

Protein N-terminal is the start point of the expression of a protein. It also influences the subcellular distribution, degradation, and the turnover rate of a protein. Thus, sequence analysis of the N-terminal of a protein is very important for studying the function of a protein. Aimed to provide comprehensive sequencing service, MtoZ Biolabs has developed a sophisticated N-T sequencing platform consisting of and protein N-T , with their advantages complementary to each other. In this service, protein sequence is not restricted by sample types, N-terminal blockage, and PTMs. Protein N-T sequences can be accurately analyzed by , whereas, the N-terminal blockage and PTMs can be analyzed by mass spectrometry.de novo sequencing

Edman sequencing

1. Determination of N-Terminal Sequence of Proteins/Peptides/Antibodies/Vaccines

2.Analysis of PTMs

1. Edman Sequencing

(1) Electroblotted Samples

Proteins separated by SDS-PAGE should be blotted onto PVDF membrane, nitrocellulose is not recommended. PVDF membrane can be stained with Coomassie blue or Poncaue red (Sliver stain is not recommended), followed by washing with ultra-pure water. The washing steps must be repeated several times, when glycine-buffer is used.

(2) Liquid Samples

1-10 ug amount of sample, with >90% purity. Avoid using Tris, glycine, guanidine, glycerol, sucrose, ethanolamine, SDS, Triton, X-100, Tween, and ammonium sulfate in the buffer.

2. MS Analysis

(1) Gel and liquid protein samples are acceptable.

(2) 5-10 ug protein samples are required. Sample purity should be as high as possible.

*Note

All reagents/solvent used must be of the highest purity to reduce contaminating substances. Samples should be handled with extreme caution and always in clean condition. Any source that may introduce contaminating proteins should be eliminated. Customers are welcome to contact us for detailed sample requirements before sending your samples.

1. Experiment Procedures

2. Parameters of Liquid Chromatography and Mass Spectrometer

3. MS Raw Data Files

4. Peptide Identifications and Intensity

5. Protein Identifications and Intensity

Protein Sequencing

Protein De Novo Seq

N-Terminal Sequencing

C-Terminal Sequencing

Edman Degradation

Protein Full-Length Sequencing

Protein Analysis

Protein Identification

Protein Mass Measurement

PTMs Identification

PTM Analysis

Phospho Proteomics

Acetyl Proteomics

Ubiquitin Proteomics

Glyco Proteomics

Disulfide Bond

Histone Modifications

MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.

MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.

Email: marketing@mtoz-biolabs.com