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MtoZ Biolabs - Model protein-de-novo-sequencing -Protein De Novo Sequencing
Protein sequences are critical information for study of protein function, as the amino acid sequence determines the advanced protein structures. Generally, there are two methods for protein sequence analysis, database search and de novo sequencing. Database search method identifies proteins by measuring peptide mass, and comparing to available protein databases. However, this method is unsuitable for analyzing antibodies, peptides, and proteins, of which the sequence information is either inaccessible, or not included in any databases. For these cases, de novo sequencing is the only way to decipher the protein sequence. MtoZ Biolabs uses the most advanced mass spectrometer-Orbitrap Fusion Lumos, for de novo sequencing service. Coupled with our professional bioinformatics analysis and reverse sequence confirmation, high-quality sequence analysis is guaranteed. Our de novo sequencing service is also well-suited for analyzing multiple kinds of antibodies, including IgM and IgG antibody subtypes, and antibodies modified with FITC, biotin, and Alexa, etc.
1. , of which Databases are UnavailableSequence Analysis of Protein/Peptide
2. of Protein/Peptide, of which Databases are UnavailablePTMs Analysis
3. Sequence Analysis of CDR Region orFull-Length of Antibodies
In this study, the full-length sequence of a monoclonal antibody is analyzed by our de novo sequencing system. MS/MS data in Fig. 1 indicated that complete fragmentation of peptide precursors and L/I residues can be easily discriminated after second collision. We used four enzymes (indicated by different color in Fig. 2) for protein digestion. Peptide mapping results showed complete sequence coverage with CDR being identified by at least 10 times, reaffirming accuracy of our sequence analysis.
1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. MS Raw Data Files
4. Peptide Identifications, Intensity, and Peptide Mapping
5. Protein Sequence and PTMs Data
MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.
Email: marketing@mtoz-biolabs.com