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MtoZ Biolabs - Model protein-isoelectric-point-determination -Protein Isoelectric Point Determination Service
Proteins are ampholytes that contain both positively and negatively charged functional groups. Their net charge is predominantly determined by the pH of the surrounding environment. When the pH of the external environment makes the acidic and alkaline dissociations in the protein molecule balance, i.e., the positive and negative charges carried by the molecule are completely canceled out, this particular pH is known as the isoelectric point (pI) of that protein. At the pI, the protein's surface charge is zero, resulting in minimal solubility. The pI is specific to each protein and depends on its composition and structure, making its accurate determination a crucial method for protein identification. Furthermore, the pI serves as a critical metric in various biochemical and proteomic analyses, including two-dimensional gel electrophoresis (2D-PAGE), X-ray crystallography, and liquid chromatography-mass spectrometry (LC-MS).
Based on capillary isoelectric focusing (cIEF), MtoZ Biolabs offers precise determination of the pI of any protein. Compared to traditional slab gel methods, cIEF boasts advantages such as short runtime, high precision, and minimal sample consumption. This technology is applicable to a variety of amino acids, peptides, recombinant proteins, enzymes, monoclonal antibodies, and has been widely utilized in clinical analysis.
Santhi, O. K. et al. Indo Am. J. P. Sci. 2021.
Protein sample concentration > 0.5 mg/mL, purity > 90%, minimum quantity not less than 50 pmol.
In the technical report, MtoZ Biolabs will provide you with a detailed report, including:
1. Experimental Procedures
2. Relevant Data
MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.
Email: marketing@mtoz-biolabs.com