MtoZ Biolabs - Model protein-secondary-structure-analysis -Protein Secondary Structure Analysis Service

Protein secondary structure analysis refers to the study of local spatial structures between continuous amino acid residues in proteins, mainly including α-helix, β-sheet, and others. Understanding protein secondary structures helps reveal their folding mechanisms, stability, function, and interactions with other biomolecules, providing important information for studying the biological functions and regulatory mechanisms of proteins. The principles of protein secondary structure analysis mainly rely on the specific responses of different structural elements (such as α-helix and β-sheet) to signals like light and magnetism. Common methods include circular dichroism (CD), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), and X-ray crystallography.

MtoZ Biolabs offers a one-stop protein secondary structure analysis service using JASCO circular dichroism spectrometer, professionally determining the spatial configuration of proteins, including α-helix, β-sheet, and random coils, etc. Through the proportion and distribution information of protein sequence's secondary structure, the composition and changes of protein secondary structure are accurately revealed.

1. Sample Preparation

Collect purified protein samples, remove impurities, adjust protein concentration and buffer to meet subsequent analysis requirements.

2. Spectrometer Calibration

Use a set of standard samples to calibrate the spectrometer, ensuring accurate measurement of the protein sample's CD spectra.

3. Spectra Collection

Place the protein sample in an optical cuvette and measure and collect CD spectra at different wavelengths (typically between 190-250 nm).

4. Data Processing and Analysis

Use professional software to analyze the measured CD spectra and convert them into proportions of protein secondary structures.

5. Results Interpretation

Interpret the protein's secondary structures based on the analysis results, assessing its folding degree, stability, and structural features.

1. Protein Structure and Function Research

Analyzing protein secondary structures can reveal functional and structural characteristics of specific protein families, aiding in understanding the mechanisms of protein function and their relationship with structure.

2. Protein Folding Research

Secondary structure analysis helps understand key intermediates in the protein folding process, thus revealing the folding mechanisms of proteins.

3. Protein Interaction Research

Analyzing protein secondary structures can enhance understanding of protein interaction mechanisms, providing a basis for biological processes and signal transduction studies.

4. Drug Design and Screening

Analyzing protein secondary structures can provide important information on the structural characteristics of drug targets, assisting researchers in designing and screening more specific and efficient drugs.

In the technical report, MtoZ Biolabs will provide you with a detailed technical information, including:

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Protein Secondary Structure Analysis

4. Mass Spectrometry Images

5. Raw Data

MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.

MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.

Email: marketing@mtoz-biolabs.com