MtoZ Biolabs - Model multipath-protein-phosphorylation -Multipathway Phosphoproteomics Service

Protein phosphorylation is a reversible post-translational modification where amino acid residues in proteins are phosphorylated by kinases, which attach covalently bonded phosphate groups. This modification alters proteins' conformation, potentially activating, deactivating, or changing their functions. Research into protein phosphorylation enhances our understanding of biological processes and helps to define the mechanisms of diseases linked to abnormal phosphorylation levels. Due to the low concentration and broad dynamic range of phosphorylated proteins in biological samples, enrichment of phosphorylated peptides is essential before performing quantitative proteomics to increase the detectability of these modifications.

MtoZ Biolabs employs the Multi-Pathway Enrichment Kit from CST company, along with Thermo Fisher's Orbitrap Fusion Lumos mass spectrometer and nanoLC, to offer comprehensive services in multipath protein phosphorylation proteomics. Clients need only specify their research goals and submit their samples; MtoZ Biolabs manages all subsequent steps including protein extraction, digestion, phosphorylated peptide enrichment, peptide separation, mass spectrometric analysis, analysis of raw data, and bioinformatics.

1. For Tissue Samples

Ship on dry ice; minimum requirements are 200 mg for plant tissues, 1 mL for blood (plasma should be anticoagulated with EDTA), 0.5 mL for serum, 2 mL for urine, 1 g for animal tissues, and 5*10 cells for cell samples. Yeast and microorganisms should have a dry weight of 200 mg.7

2. For Protein Samples

Ensure a minimum of 1 mg total protein. Use standard lysis solutions for tissue and cell extraction.

3. Sample Shipping

Transport samples with adequate dry ice and opt for expedited shipping to minimize degradation risks during transit.

4. Sample Testing

We evaluate all samples before commencing the actual experiments. Testing must confirm sample integrity before proceeding with formal analyses.

MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.

MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.

Email: marketing@mtoz-biolabs.com