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Pan-genome Microarrays Services
Pan-genome Microarrays are used to determine the genetic makeup of un-known microbial strains and can introduce insights into phylogenetic relationships. At ArrayGen, we help you to design high quality probes for Pan-genome Microarrays which would help identify genotyping of unknown microbial iso-lates.
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- Genome wide determination of the genetic makeup of an un-known microbial iso-lates
- Determine novel genomes and multiple genomes in a mixture
- Validate the gene context of an unknown genome from re-sequencing next generation sequencing (NGS) data using microarrays
- Arrays designed according to gene contexts of an unknown genome of interest to the customer
- Cluster generation using CD-Hit and our own in-house propriatory tool
- 60-mer probe length
- Probe selection based on %TM, %GC, PolyX, 3` end, self hybridization, nonoverlaping and high hybridization specificity
- Removal of probes in repeat-masked and vector-masked regions
- In silico hybridization check for each probe by BLASTN
- We design one probe per target ( Core, Accessory and Unique ) regions. In case you would like to add more than one probe for each target, please let us know.
- We also include additional probes for technical QC
- Control probes are added based on platform specific protocol. In case you would like to add your own control probes, please let us know.
- Complete annotation of designed probes for fast and easy data interpretation
- High confidence in the design of your arrays
- We focus on quality assurance followed by quality control process of array design
- Maximum flexibility in array content and format to identify genome-wide expression levels of thousands of genes
- Ensures highest level of coverage, uniformity, specificity and sensitivity of your arrays
- Quick turnaround time from 3 days to a week
- Cost-effective formats for your array designing
- Complete support on array designing services