MtoZ Biolabs - Model principal-component-analysis -Principal Component Analysis (PCA) Service
Principal component analysis (PCA) is the linear recombination of all metabolites identified to form a set of new comprehensive variables, and select 2-3 variables, reflecting as much information of the original variables as possible, based on the analyzed problem to achieve the purpose of dimension reduction. At the same time, PCA of metabolites can also reflect the variability between and within groups overall. The total sample PCA analysis uses the PCA method to observe the overall distribution trend between all group samples, identify potential outlier samples, and consider various factors (number of samples, preciousness of samples, dispersion degree) to decide whether to remove outlier points. The PCA score chart for all samples is shown below (PCA score chart for pairwise analysis of samples).
MtoZ Biolabs uses XCMS software to extract ion peaks of metabolites. The peaks extracted from 25 samples and QC samples, after normalization, undergo PCA analysis. As shown in the chart, QC samples (grey) are closely clustered together, indicating good stability of the instrument analysis system in this experiment, reliable experimental data. The metabolic spectrum differences obtained in the experiment can reflect the biological differences between samples.
MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.
Email: marketing@mtoz-biolabs.com