MtoZ Biolabs - Model protein-full-length-sequencing -Protein Full-Length Sequencing
Full-length sequence information is essential for studying the integrity and correct translation of biopharmaceuticals. In general, protein sequence analysis service provided by MtoZ Biolabs includes three steps: digestion of proteins into smaller peptide fragments; tandem LC-MS/MS analysis of peptides; MS/MS data analysis. Traditional protein sequencing only uses trypsin for digestion, which renders the final protein sequence coverage of around 60%. To ensure 100% protein sequence coverage, MtoZ Biolabs uses up to 6 kinds of protein enzymes for protein digestions, eliminating any missing peptides.biopharmaceuticals
1. Determination of Full-Length Sequence of Proteins/Peptides/Antibodies/Vaccines
2. & Chemical Modifications on ProteinAnalysis of PTMs
3. Verification of the Correct Translation of Recombinant Proteins
In this project, we analyzed the full-length sequence of an antibody product sent from one of our customers. To ensure 100% sequence coverage, we selected 4 enzymes for protein digestion, based on the predicted antibody sequence. The sequence mapping results showed 100% sequence coverage.
1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. MS Raw Data Files
4. Peptide Identifications and Intensity
5. Protein Identifications and Intensity
MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.
Email: marketing@mtoz-biolabs.com