MtoZ Biolabs - Model shotgun -Protein Identification Service by Shotgun Proteomics

Shotgun proteomics, also known as a shotgun method, refers to the utilization of bottom-up proteomics techniques to study all proteins present in complex mixtures such as serum, urine, and cell lysates. It combines high performance liquid chromatography (HPLC) and mass spectrometry (MS). The uniqueness of shotgun proteomics lies in its ability to identify and quantify multiple proteins while minimally separating them.

The goal of shotgun proteomics analysis is to identify as many proteins as possible in the sample. Shotgun proteomics analysis utilizes shotgun techniques for comprehensive protein analysis. The basic method involves enzymatic digestion of proteins in the mixture, followed by liquid chromatography (LC) separation of peptides and subsequent identification of peptides using tandem mass spectrometry (MS/MS) to achieve protein identification. Shotgun proteomics analysis allows for the identification of all proteins in the sample, simultaneously identifying hundreds or thousands of proteins.

MtoZ Biolabs shotgun proteomics identification service is based on ultra-high-resolution mass spectrometry technology, utilizing Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform combined with nanoLC-MS/MS nanoscale chromatography, enabling comprehensive identification of a large number of proteins in whole cells, tissues, or complex mixed samples.

1. Enables Large-scale and Global Protein Identification and Quantification

2. Allows for Comparative Analysis of Different Samples

3. Ideal Choice for Discovery and Research of Protein Biomarkers

4. Applicable to Dynamic Proteomics Analysis

1. Identification and Quantification of Individual Proteins

2. Identification and Quantification of Proteins in Complexes

3. Identification and Quantification of Post-translational Modifications

4. Analysis of Protein Interaction

1. Liquid Chromatography (LC)

2. High Performance Liquid Chromatography (HPLC)

3. Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS)

4. Electrospray Ionization Mass Spectrometry (ESI-MS)

In the technical report, MtoZ Biolabs will provide you a detailed information, including:

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Mass Spectrometry Images

4. Raw Data

5. Detailed Information on Identified Proteins

MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.

MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.

Email: marketing@mtoz-biolabs.com