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MtoZ Biolabs - Model protein-lactylation-modification -Protein Lactylation Modification Analysis Service
In cell biology and biochemistry, post-translational modifications (PTMs) critically influence the stability, activity, localization, and functions of proteins. Among these, lactylation stands out as a significant PTM. This modification can occur on any protein residue with a free amino group, such as lysine, arginine, and tyrosine. During this process, lactate reacts with these amino acids to form an ester linkage, thereby altering protein structure and function. This change impacts several biological processes, including cell signaling, gene expression, and metabolism. Accurate identification and characterization of lactylation sites are essential for understanding their biological implications and for the development of related applications.
Mass spectrometry is crucial for identifying lactylated proteins and studying their properties. This technique allows for precise determination of lactylation sites and explores the modification's impact on protein structure and function. Additionally, mass spectrometry is also used to screen for new lactylation sites, providing deeper insights into protein lactylation studies. Moreover, research on lactylation modification has a broad application value, including but not limited to understanding the structure and function of proteins, studying the pathogenesis of diseases, and the development of novel drugs, and helping us understand the complexity and dynamics of biological systems.
Wu, X. et. al. Nat. Methods. 2022.
At MtoZ Biolabs, using the latest Thermo's Obitrap Exploris 240 mass spectrometer in combination with Nano-LC technology, we have developed a precise analytical platform for protein lactylation. This platform is adept at pinpointing lactylation sites and assessing their dynamic changes under various conditions, thereby revealing their effects on protein structure and function. Whether you aim to investigate the fundamental mechanisms of lactylation or its role in disease progression, MtoZ Biolabs offers comprehensive, efficient, and tailored analytical solutions. Contact us for more information on our protein lactylation modification analysis services.
1. Protein Sample Preparation
Extract proteins from biological samples.
2. Protein Digestion
Digest proteins into smaller peptides using enzymes like trypsin or chymotrypsin.
3. Liquid Chromatography Separation
Further separate enriched peptides via high-performance liquid chromatography.
4. Peptide Enrichment
Enrich lactylated peptides using techniques like antibody enrichment or specific solid phase extraction.
5. Mass Spectrometric Analysis
Determine peptide masses and their fragmentation patterns to identify amino acid sequences, lactylation sites, and obtain quantitative data.
1. Sensitivity and Accuracy
Detect lactylation modifications with high precision even at low abundances.
2. Localization of Modification Sites
Identify lactylation on proteins and precisely determine the affected amino acid residues.
3. Quantitative Capabilities
Assess the relative or absolute abundance of lactylation modifications to understand their biological impacts.
4. High-throughput Capability
Identify and quantify numerous lactylation sites in a single experiment.
5. Compatibility
Combine with other techniques like immunoprecipitation and bioinformatics for more comprehensive analyses.
In the technical report, MtoZ Biolabs will provide you with detailed technical information, including:
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Information on Protein Lactylation Modifications
4. Mass Spectrometry Images
5. Raw Data
MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.
Email: marketing@mtoz-biolabs.com