MtoZ Biolabs - Model therapeutics-peptide-mass-fingerprinting -Protein Spectrometric Analysis Service

MALDI-TOF analysis of peptide mixtures is capable of tolerating moderate amounts of buffers and salts, with peptide fragments predominantly producing singly charged ions, making MALDI-TOF MS an ideal tool for peptide mass mapping. To confirm the theoretical sequence of a protein/polypeptide sample using MALDI-TOF, enzymatic digestion is first performed on the sample, followed by the verification of the peptide mass fingerprinting (PMF) via MALDI MS. Subsequent MS/MS analysis is used to validate spectral peaks, which are then compared against your provided theoretical sequence to generate a peptide coverage mapping. MtoZ Biolabs leverages its MS technology platform to develop analyses based on MALDI-TOF MS.

1. Advantages

This method is a classic approach to protein identification, characterized by its simplicity, speed, and broad application prior to the emergence of tandem mass spectrometry.

2. Limitations

Peptides of similar mass increase the difficulty of matching and cannot achieve the identification of mixed proteins. It is not suitable for protein identification in species with incomplete databases, cannot analyze post-translational modification sites, and is not easily connected with liquid chromatography.

In the technical report, MtoZ Biolabs will provide you with detailed technical information, including:

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Mass Spectrometry Images

4. Raw Data

5. Peptide Mapping Information

MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.

MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.

Email: marketing@mtoz-biolabs.com