Proteomics - Proteome Mapping Services

Pure protein sample is digested and run through an extended 1- or 2-D LC gradient, and the eluent injected into a LCMS instrument.  MS spectra are analysed to identify proteins of interest.

Outcomes of this protein analysis are dependent on the quantity and range of proteins present. A 1D LC-MS/MS analysis will identify >1000 proteins. If the sample is complex (e.g whole cell lysate) a more sophisticated 2D LC-MS/MS protocol is used. The sample is first separated by strong cation exchanged chromatography, followed by reverse-phase chromatography and analysed by 2D LC-MS/MS which can identify >2000 proteins in one experiment.

Request Form: Proteome Mapping

For more information please refer to PI publications:

• Deep Proteogenomics
• Proteomic Analysis of the Venom