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This evaluation has been performed in the laboratory of Jyväskylä Professional University, Dep. of Technology and Logistics, Rajakatu, Jyväskylä.
The first 35 tests (in the beginning of July) were intended in the optimization of testing equipment (Figure 1).
Following values of main parameters were then chosen :
- Air pressure for spraying of the microbial suspension from ”Spira” (est. 10% of the constant output pressure in the laboratory)
- ATP concentrations (related to cell counts) of bacterial suspensions (200 +/-50 RLU)
- Length of spraying period (3 min)
- Length of sampling period (1 min)
- Length of chamber cleaning by continuous flow of air between trials (min. 10 min)
The densities of bacteria in suspensions for aerosol generation with ”Spira” were controlled with luminometric ATP Assay (BIOORBIT 1253 Luminometer by BIO-ORBIT Oy, Finland, and ATP Biomass Kit HS by BIOTHEMA AB, Sweden).
Densities of fungal spores in suspensions were analyzed with microscopical countings, respectively. Certain variation of densities were needed for statistical purposes but ATP values of bacteria were limited inside a range of tens to hundreds RLU units and count of spores inside a range of tens per field (with 100* objective and 10* ocular) in every case.