Single-Particle cryo-EM is an electron microscopy method used to determine structures of individual proteins and other macromolecular complexes. Structures of macromolecules can be determined from cryo-EM images at near-atomic and sometimes true atomic resolution.
Single-Particle cryo-EM can be used to answer specific questions concerning protein-protein interactions and identify protein/domain assembly. Antibodies or Fab can be visualized when bound to their antigen. Besides, the combination of cryo-EM and AI provides an opportunity to be the new direction of future development of cryo-EM. The rapid development of cryo-EM will make it as an indispensable part of modern drug discovery.
