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Wastewater Surveillance Testing for Infectious Agents
Explore tools and resources for wastewater surveillance testing. Wastewater surveillance is a useful tool for monitoring the regional spread of infectious diseases before an outbreak occurs. With the emergence of SARS-CoV-2 infections, multiple organizations worldwide are working on SARS-CoV-2 surveillance methods using wastewater to monitor regional or city-level spread of the virus, analyze individual dorms on campuses to identify asymptomatic carriers, capture emerging mutations, and respond to possible infections. Methods of detection and surveillance for SARS-CoV-2 are outlined in this webpage and can potentially be adapted for all pathogens, including viruses, bacteria, and fungi.
Wastewater samples must be concentrated and then extracted for improved detection and analysis. Concentration steps can range from precipitation, ultracentrifugation, to the most efficient and advanced, virus enrichment through Dynabeads.
KingFisher instruments paired with MagMAX wastewater kits allow for a completely automated nucleic acid isolation format without inconvenient centrifugation, filtration, or bead beating steps.
From the eluate containing concentrated viral particles, viral nucleic acid can be purified using the Applied Biosystems MagMAX Wastewater Ultra Nucleic Acid Isolation Kit. This kit allows for low-volume (1 mL), medium volume (10 mL), and high volume (50 mL) sample extraction.