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FUJIFILM Wako Yokogawa - Version CPf -Cell Pathfinder
Designed with both screeners and microscopists in mind, CPf utilizes simple application templates for standard assays such as Cell Cycle and Translocation, a simplified basic mode for users who are new to high content analysis, and an advanced mode with over 100 selections to optimize object identification. All modes are designed with a flexible and straightforward workflow-based interface that makes the entire process simple but powerful.
Live Cell Long Term Time Lapse: image a plate for 3+ days while maintaining healthy cells utilizing the CV8000’s CO2, Temperature and Active Humidification
- CPf’s Cell Tracking module provides 9 parameters users can adjust to ensure the same object is consistently tracked frame to frame
- Track cell division. Output links mother cells to daughter cells
- Track speed, distance moved, Linearity of Motion
Live Cell High Speed Time Lapse image at almost 50fps and add inhibitors/activators in real time using the CV8000’s built in disposable tip pipettor
- CPf’s Time in Same Region model creates a maximum image projection across time. This enables CPf to create a single fixed area to measure changes over the course of a high speed time lapse assay.
- Run Ca+ assays, cardiomyocyte assays, and more
3D Imaging: build 3D models of your cells, pull out more information, and expand the knowledge of your biological system
- Report volume, surface area, and count objects within spheroids, organoids, or PDX tumor models.
- Analyze in 3D or convert to maximum, minimum, average, or sum image projection
SearchFirst™ : quickly capture rare events, improve imaging speed, and locate spheroids
- First Scan using low powered objectives then acquire objects of interest at high resolution
Machine Learning and Manual Controls : Utilize the machine learning module to teach CPf how to distinguish objects of interest from each other and from background.
- Extremely powerful tool for ill-defined objects
- Manually trace objects of interest or regions of interest to ensure only those objects important to the experiment are being captured.
Texture Analysis : utilize texture analysis to identify differences between cell states that may not be apparent when using standard intensity-based output.
- Report Peak, Valley, Hole, Edge, Ridge, and Saddle textures
- Control pixel distance, test and compare results
Gating : separate cell with distinct characteristics into populations using the gating module