Integrated DNA Technologies, Inc.
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OligoAnalyzerOligo Design & Handling Tools

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Quickly see GC content, melting temperature, and more. Identify secondary structure potential. Minimize dimerization. Use NCBI Blast. 

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ENTER YOUR SEQUENCE

  • DNA
  • RNA
  • Locked nucleic acid bases
  • Modifications
  • Mixed bases
  • Phosphorothioated bases
  • Icons Library_Sky_85x85_Amplification

ADJUST CALCULATION OPTIONS

  • Hybridization target type
  • Oligo concentration
  • Na+ concentration
  • Mg2+ concentration
  • dNTP concentration
  • Use SpecSheet or qPCR preset values
  • Icons Library_Sky_85x85_How to order

CHOOSE A FUNCTION

  • Standard analysis
  • Hairpin
  • Self-dimer
  • Hetero-dimer
  • NCBI blast
  • Tm mismatch