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Intro for TEER Assay Article From Cellqart
May. 31, 2021
Courtesy ofSterlitech Corporation
How often do you or your colleagues perform TEER (Transepithelial-Transendothelial Electrical Resistance) assays in a given week, and how confident are you in your results?
The accuracy of your TEER assay measurements depends on four things:
- Temperature - It’s recommended you perform yours at exactly 37°C.
- Electrode positioning - The lower chamber electrode must touch the bottom of the well and the upper chamber electrode must not touch the membrane.
- Vibrations - Vibrations can alter electrode placement and they cause disturbance of the electric field across the monolayer
The 4th factor is membrane quality, and there’s now a cell culture insert membrane that undergoes 100% in-line quality control of every segment, making it a revolutionary upgrade to your lab’s TEER assay workflow.
Decide for yourself if it’s right for you and your colleagues.
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