155 Articles found
American Society for Microbiology (ASM) Articles
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Extracellular signal-regulated kinase 2 (ERK2) phosphorylation sites and docking domain on the nuclear pore complex protein Tpr cooperatively regulate ERK2-Tpr interaction
Identifying direct substrates of mitogen-activated protein kinases (MAPKs) and understanding how those substrates are selected is central to understanding how these ubiquitously activated enzymes generate diverse biological responses. In previous ...
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Sir3-nucleosome interactions in spreading of silent chromatin in saccharomyces cerevisiae
Silent chromatin in Saccharomyces cerevisiae is established in a stepwise process involving the SIR complex, comprised of the histone deacetylase Sir2 and the structural components Sir3 and Sir4. The Sir3 protein, which is the primary ...
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Molecular architecture of quartet MOZ/MORF histone acetyltransferase complexes
The monocytic leukemia zinc finger protein MOZ and the related factor MORF form tetrameric complexes with ING5 (inhibitor of growth 5), EAF6 (Esa1-associated factor 6 ortholog), and the bromodomain-PHD finger protein BRPF1, -2, or -3. To gain new ...
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Brominated furanones inhibit biofilm formation by salmonella enterica serovar typhimurium
Salmonella enterica serovar Typhimurium is a main cause of bacterial food-borne diseases. As Salmonella can form biofilms in which it is better protected against antimicrobial agents on a wide diversity of surfaces, it is of interest to explore ...
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Mechanistic dissection of the enzyme complexes involved in biosynthesis of lacticin 3147 and nisin
The thioether rings in the lantibiotics lacticin 3147 and nisin are posttranslationally introduced by dehydration of serines and threonines, followed by coupling of these dehydrated residues to cysteines. The prepeptides of the two-component ...
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Metagenomic screening for bleomycin resistance genes {triangledown}
A metagenomic library of activated sludge was screened for bleomycin resistance genes. Two genes were identified that differed greatly from each other, from the genes of bleomycin-producing actinomycetes, and from those of clinical isolates. ...
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Drosophila maternal Hsp83 mRNA destabilization is directed by multiple SMAUG recognition elements in the open reading frame
SMAUG (SMG) is an RNA-binding protein that functions as a key component of a transcript degradation pathway that eliminates maternal mRNAs in the bulk cytoplasm of activated Drosophila melanogaster eggs. We previously showed that SMG destabilizes ...
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Identification, cloning, and characterization of a novel ketoreductase from the cyanobacterium synechococcus sp. Strain pcc 7942
A new ketoreductase useful for asymmetric synthesis of chiral alcohols was identified in the cyanobacterium Synechococcus sp. strain PCC 7942. Mass spectrometry of trypsin-digested peptides identified the protein as ...
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Mutational analysis of the class iia bacteriocin curvacin a and its orientation in target cell membranes
To analyze the orientation in target cell membranes of the pediocin-like bacteriocin (antimicrobial peptide) curvacin A, 55 variants were generated by site-directed mutagenesis and their potencies against four different target cells determined. ...
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Type ii hydride transferases from different microorganisms yield nitrite and diarylamines from polynitroaromatic compounds
Homogenous preparations of XenB of Pseudomonas putida, pentaerythritol tetranitrate reductase of Enterobacter cloacae, and N-ethylmaleimide reductase of Escherichia coli, all type II hydride transferases of the Old Yellow Enzyme family of ...
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Host ranges of listeria-specific bacteriophages from the turkey processing plant environment in the united states
Even though at least 400 Listeria phages have been isolated from various sources, limited information is available on phages from the food processing plant environment. Phages in the processing plant environment may play critical roles in ...
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Subfunctionality of hydride transferases of the old yellow enzyme family of flavoproteins of pseudomonas putida
To investigate potential complementary activities of multiple enzymes belonging to the same family within a single microorganism, we chose a set of Old Yellow Enzyme (OYE) homologs of Pseudomonas putida. The physiological function of these ...
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Evaluating the insecticidal genes and their expressed products in bacillus thuringiensis strains by combining pcr with mass spectrometry
By a combination of PCR and mass spectrometry, a total of five cry genes (cry1Aa, cry1Ac, cry2Aa, cry2Ab, and cry1Ia) were detected in genomic DNA from the wild-type Bacillus thuringiensis strain 4.0718, and three protoxins (Cry1Aa, Cry1Ac, and ...
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Soil formate regulates the fungal nitrous oxide emission pathway
Fungal activity is a major driver in the global nitrogen cycle, and mounting evidence suggests that fungal denitrification activity contributes significantly to soil emissions of the greenhouse gas nitrous oxide (N2O). The metabolic pathway and ...
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Disrupting vesicular trafficking at the endosome attenuates transcriptional activation by Gcn4
The late endosome (MVB) plays a key role in coordinating vesicular transport of proteins between the Golgi complex, vacuole/lysosome, and plasma membrane. We found that deleting multiple genes involved in vesicle fusion at the MVB (class C/D vps ...
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Mechanistic insights into replication termination as revealed by investigations of the Reb1-Ter3 complex of schizosaccharomyces pombe
Relatively little is known about the interaction of eukaryotic replication terminator proteins with the cognate termini and the replication termination mechanism. Here, we report a biochemical analysis of the interaction of the Reb1 terminator ...
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Stb1 collaborates with other regulators to modulate the G1-specific transcriptional circuit
G1-specific transcription in the budding yeast Saccharomyces cerevisiae depends upon SBF and MBF. Whereas inactivation of SBF-regulated genes during the G1/S transition depends upon mitotic B-type cyclins, inactivation of MBF has been reported to ...
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P53-targeted lsd1 functions in repression of chromatin structure and transcription in vivo
Despite years of study focused on the tumor suppressor p53, little is understood about its functions in normal, differentiated cells. We found that p53 directly interacts with lysine-specific demethylase 1 (LSD1) to alter chromatin structure and ...
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Two subunits specific to the pbap chromatin remodeling complex have distinct and redundant functions during drosophila development
Chromatin remodeling complexes control the availability of DNA binding sites to transcriptional regulators. Two distinct conserved forms of the SWI/SNF class of complexes are characterized by the presence of specific accessory subunits. In ...
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Distinct functions of pot1 at telomeres
The mammalian protein POT1 binds to telomeric single-stranded DNA (ssDNA), protecting chromosome ends from being detected as sites of DNA damage. POT1 is composed of an N-terminal ssDNA-binding domain and a C-terminal protein interaction domain. ...