Applied Scientific Instrumentation products

The Single-Objective Light Sheet Microscope from ASI, developed in collaboration with Leica Microsystems, revolutionizes 4D fluorescence imaging by integrating illumination and detection within a single sample-facing objective. The system is known in scientific literature as OPM, SCAPE, SOPi, eSPIM, and SOLS, and offers high-speed imaging with minimal light exposure. This microscope utilizes galvo scanning for simultaneous light sheet illumination and detection plane scanning, allowing volumetric imaging at frame rates limited only by camera capabilities. It is noted for negligible photobleaching and superb optical sectioning. The solution is compatible with standard sample holders like 35 mm dishes and multi-well plates, as well as various laser launches and sCMOS cameras. Designed for imaging cells and organoids, the system is customizable, versatile, and cost-effective, catering to diverse scientific research needs in cell biology and neuroscience.

The CRISP Autofocus System is intricately designed to maintain precise focus during microscopy operations. It compensates for thermal variations and other factors that can lead to sample drift during prolonged observations. When integrated with the Dual C-Mount Splitter (DCMS), it provides a robust focus stabilization solution that supports both analog and high-resolution digital camera inputs. CRISP detects focus shifts by projecting an IR LED illuminated mask, capturing the focus error through lateral motion detection. This enables closed-loop control for focus adjustment. It is typically mounted with ASI's Dual C-Mount Splitter, housing the necessary dichroic beam combiner and blocking filters. Note that CRISP relies on reflected light from sample interfaces with varying refractive indices and is not suitable for index matching mounts. Its specifications delve into adjustments and options, enhancing its versatility for sophisticated microscopy applications.

The XYZ Tracker is an advanced system utilized for automated imaging of freely moving or semi-restrained model organisms such as C. elegans, drosophila larvae, and larval zebra fish. It plays a critical role in studies involving behavioral genetics, neuronal activity linkage to behaviors, and responsive stimuli probing. The tracker maintains the specimen within the field of view and in focus by utilizing ASI’s Tunable Lens and a secondary camera. This setup captures images from multiple focal planes without moving the objective or sample, adjusting the XYZ axes automatically as needed. It includes a software plug-in and integrates with ASI's XY and Z stages. The system leverages Micro-Manager, an open-source imaging solution supporting a wide range of cameras and microscopes, to control and adjust components effectively. The integration of tunable lenses, C-mounts, and robust tracking mechanisms makes it optimal for intricate imaging tasks in scientific research.

Objective adapter for Nikon M25-0.75 threads (NIKON CFI 60 series objectives).

The Modular Infinity Microscope System by ASI is a highly adaptable solution designed for specialized microscopy applications. It employs a versatile system of tube lenses and adapters, which facilitate seamless integration of various components, including illumination and imaging modules. The unique design allows for the use of infinity space, permitting the addition of supplementary optical systems without altering magnification. This flexibility is critical for applications involving epi-fluorescent illumination, where a dichroic beam splitter and filter cube can be employed. The system's component assembly utilizes a standard 38 mm diameter coupling ring, ensuring robust and precise construction. This microscope system can be easily customized with essential components such as a microscope objective, tube lens, and camera mount. The MIM system enables researchers to construct single or double-layer microscopes and integrate automated focus mechanisms. Additional features include cube-based and CUBE-SLDR microscopes enhancing focus travel and integration with the LS-50 stage.