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ASI - Single-Objective Light Sheet Microscope for 4D Fluorescence Imaging
The Single-Objective Light Sheet Microscope from ASI, developed in collaboration with Leica Microsystems, revolutionizes 4D fluorescence imaging by integrating illumination and detection within a single sample-facing objective. The system is known in scientific literature as OPM, SCAPE, SOPi, eSPIM, and SOLS, and offers high-speed imaging with minimal light exposure. This microscope utilizes galvo scanning for simultaneous light sheet illumination and detection plane scanning, allowing volumetric imaging at frame rates limited only by camera capabilities. It is noted for negligible photobleaching and superb optical sectioning. The solution is compatible with standard sample holders like 35 mm dishes and multi-well plates, as well as various laser launches and sCMOS cameras. Designed for imaging cells and organoids, the system is customizable, versatile, and cost-effective, catering to diverse scientific research needs in cell biology and neuroscience.
The single-objective light sheet geometry – known in the literature as OPM, SCAPE, SOPi, eSPIM, and SOLS – enables 4D fluorescence imaging of biological samples at high speed and low light dose with conventional sample mounting. Previously such a microscope had to be home-built; now ASI is making this transformative technology broadly available in collaboration with Leica Microsystems.
The single sample-facing objective is used for both light sheet illumination and detection. The illumination light sheet and detection plane are scanned together at the sample using a galvo, and the stationary descanned image is captured by a camera. This scheme allows volumetric imaging at camera-limited frame rates with negligible bleaching and excellent optical sectioning.
This microscope system is flexible, affordable, and easily customized to fit your needs. It is compatible with most laser launches and sCMOS cameras. It is well-suited to image cells, organoids, or similar cell cultures mounted in any coverslip-bottom holder including 35 mm dishes, chamber slides, or multi-well plates.
Single-objective light sheet features
- flexible and customizable to fit various user needs
- affordable compared with alternative systems
- well-suited to image cells, organoids, or cell cultures
- compatible with well plates, 35 mm dishes, and chamber slides
- compatible with most sCMOS cameras and laser launches
- camera-limited frame rates and with millisecond flyback for fast volumetric imaging
- compatible with a range of environmental control options
Single-objective light sheet configurations
- “CB1” configuration with Leica HC PL APO 40x/1.10 W CORR primary objective
- “NS1” configuration with 20x/1.0 W primary objective in either inverted or upright configuration
- Simultaneous multi-channel detection
- Water dispenser
- Environmental control
- Hardware autofocus
- Path for photomanipulation