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biotechrabbit GmbH products
PCR Products - Standard PCR
Taq DNA Polymerase, 5 U/µl
biotechrabbit™ Taq DNA polymerase is a first-choice enzyme for all routine and molecular diagnostics PCR applications . The exceptional quality and purity of the enzyme ensures highest performance that is utilized by the diagnostics industry and research labs. The polymerase is suitable for standard and fast PCR applications giving high product yields from various templates with targets of up to 3 kb in size. Taq DNA polymerase is a thermostable, highly processive 5`→3` DNA polymerase that has low 5`→ 3` exonuclease activity and lacks 3`→5` exonuclease (proofreading) activity. The latter allows incorporation of modified nucleotides. The enzyme also exhibits deoxynucleotidyl transferase activity that results in the addition of extra A overhang at the 3`-ends of PCR products, allowing easy cloning of PCR products into vectors with T overhangs.
2X YourTaq™ PCR Master Mix
biotechrabbit™ YourTaq PCR Master Mix is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. The mix shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq PCR Master Mix is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors. The 2X YourTaq PCR Master Mix contains pure biotechrabbit YourTaq DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added. In addition, the mix is suitable for amplification of GC-rich templates (up to 70%) pairing with 5X PCR Enhancer (BR1900201).
Lyophilized PCR Kits
5X CAPITAL™ 1-Step qRT-PCR Probe Master Mix, lyophilized
biotechrabbit™ lyophilized CAPITAL 1-Step qRT-PCR Probe Master Mix is a freeze-dried version of the well-established liquid equivalent. The stabilized format allows shipment and storage without cooling. The master mix is optimized for real-time PCR quantification of RNA templates, including mRNA, total RNA and viral RNA from a wide range of targets. The mix ensures high specificity and sensitivity in single and multiplex detection, making it the choice for extremely low-copy-number targets in pathogen detection. CAPITAL 1-Step qRT-PCR Probe Master Mix uses proprietary reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and QPCR in a single tube.
4X CAPITAL™ qPCR Probe Master Mix, lyophilized
biotechrabbit™ lyophilized CAPITAL qPCR Probe Mix is a freeze-dried version of the well-established liquid equivalent. The stabilized format allows shipment and storage without cooling. The master mix is optimized for quantifying genomic, cDNA and viral sequences provides outstanding performance in single and multiplex qPCR. The high sensitivity provided by the mix is ideal for detection of low-abundance DNA targets in various applications, such as pathogen detection. CAPITAL qPCR Probe Mix uses proprietary combination of enzyme and buffer chemistry for efficient extension and early Ct in single and multiplex qPCR.
Real Time PCR - CAPITAL qPCR Mixes
4X CAPITAL™ qPCR Probe Master Mix
biotechrabbit™ CAPITAL qPCR Probe Master Mix for quantifying genomic, cDNA and viral sequences provides outstanding performance in single and multiplex qPCR. The high sensitivity provided by the mix is ideal for detection of low-abundance DNA targets in various applications, such as pathogen detection. CAPITAL qPCR Probe Master Mix uses proprietary combination of enzyme and buffer chemistry for efficient extension and early Ct in single and multiplex qPCR. To enable the use of the kit on qPCR platforms with different reference dye concentration requirements, three kit formats are available: a kit containing no ROX, as well as LRox and HRox versions containing ROX in the corresponding concentrations.
