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4X CAPITAL™ qPCR Probe Master Mix, lyophilized

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biotechrabbit™ lyophilized CAPITAL qPCR Probe Mix is a freeze-dried version of the well-established liquid equivalent. The stabilized format allows shipment and storage without cooling. The master mix is optimized for quantifying genomic, cDNA and viral sequences provides outstanding performance in single and multiplex qPCR. The high sensitivity provided by the mix is ideal for detection of low-abundance DNA targets in various applications, such as pathogen detection. CAPITAL qPCR Probe Mix uses proprietary combination of enzyme and buffer chemistry for efficient extension and early Ct in single and multiplex qPCR.

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  • Stable enzyme and mix for ambient shipment and room-temperature storage
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  • Highly sensitive for low-abundance DNA targets

  • Standard and fast cycling qPCR with rapid extension rate for early Ct values
  • For use on a wide range of probe technologies including Taqman®, Molecular Beacons® and Scorpion® probes

Component

Composition

Lyo CAPITAL qPCR Probe Mix

Cake of lyophilized 4× qPCR Probe Master Mix

qPCR Probe Reconstitution Buffer    

Optimized PCR buffer for reconstituting lyophilized CAPITAL qPCR Probe Mix

 

LYO Master Mix RECONSTITUTION

1) Transfer the whole content of one vial qPCR Mix Reconstitution Buffer to one vial Lyo CAPITAL qPCR Probe Mix
2) Mix well – the lyophilisate will dissolve within seconds
3) Store the reconstituted CAPITAL qPCR Probe Mix at -20°C 

STORAGE

Store at room temperature or below (until expiry date – see product label)
Reconstituted lyophilisate: store at −20°C for up to 12 months

 

 

Quality Control

Functional assay

Mix tested functionally in qPCR.