The CHALLENGE AER-200 Respirometer System consists of one or more biological reaction vessels, gas flow measuring cells, and a computer. The gas measuring base contains eight flow ceHs and associated interconnecting circuitry. As gas flows through each cell under the influence of a slight pressure caused by gas production in the reaction vessel, bubbles of a fixed volume are formed in the lower section of the cell. These bubbles in turn pass through a detection section where a counter is activated. Finally,...
METHOD: A full-scale compost plant was faced with the need to determine the stability of the compost in the active bays during the compost operation. Samples were collected at 24-ft intervals (2 days of operation) through the length of one bay of the in-vessel compost system and placed into sealed glass reactors. Air was recirculated through the compost material using a peristaltic pump. A caustic scrubber was placed in the recirculation line to remove carbon dioxide (Figure 1). The reaction vessels were...
A pharmaceutical company was interested in knowing whether a specific component, MCB, inhibited the biodegradation of other wastewater constituents. A series of respirometer tests was set up using identical mixtures of wastewater and return activated sludge (RAS) plus varying amounts of MCB. The OUR fingerprint of this wastewater includes a first peak of about 500 mg/L-hr within the first hour of contact that represents the oxidation of highly-biodegradable organic constituents. A second group of readily...
The methane production activity of anaerobic sludges is related to the history of the sludge, the biomass yield from the wastewater the sludge has been treating, the fraction of methanogens, and the presence of toxic substances.
Test Setup: 40 and 60 mL of wastewater were added to individual respirometer flasks each containing nutrient/mineral/buffer medium to form two different dilutions. TCMP was added to inhibit nitrification. 25 mL of seed from a laboratory scale reactor was added to each test reactor. Oxygen uptake was monitored using a CHALLENGE AER-200 respirometer for five days. A second dose of 40 and 60 mL of leachate sample was then added to each reactor and oxygen uptake was measured for another 5 days.
Waste Type: Complex alcohols. Objective: Certain constituents of the test waste were know to be biodegradable but were suspected of being toxic to aerobic biological processes when present in high concentrations. The objective was then to determine how much waste could be added to an activated sludge process before toxic impacts would be experienced.
OUR measurements were made for two waste streams from a chemical production plant. The fingerprint for Waste 1 indicated the presence of three major groups of organic constituents. The first group of readily biodegradable organic constituents is oxidized at maximum kinetic rate through about one hour of contact. Oxidation of a second group of constituents causes the OUR to increase through about 3.5 hours of incubation. This increase in OUR is caused by growth of microorganisms during the oxidation reaction....
Waste Type:Synthetic (Acetic Acid). Objective: To monitor the gas produced by steady-state cultures receiving acetate as the input substrate. Another objective was to verify the consistency of gas production over a period of time as a means of verifying culture stability and test repeatability.
Waste Type: Synthetic (Ethanol). Objective: To model the methane produced from acetate and from hydrogen in cultures receiving ethanol as an input substrate. The objective was to verify the accuracy of models developed on the basis of substrate conversion.