41 services found
Creative Proteomics Services
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Proteomics Service - Protein Identification
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Sample Preparation Services
Sample preparation is critical for proteomics analysis, and Creative Proteomics provides sample preparation service according to your ...
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In-gel or In-solution Digestion Services
Among the endoproteases which could be used for protein digestion, the serine protease trypsin is most commonly employed as it generates peptides which are highly amenable to MS(/MS) analysis. Depending on the preceding workflow, the enzymatic digestion of proteins is performed either in-gel or in-solution, generally, the in-gel digestion methodology has become routine for proteins separated by 2-D electrophoresis while in-solution digestion are usually ...
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Molecular Weight Determination and Molecular Weight Distribution of Peptides or Proteins
Our comprehensive service and detailed reports will provide you with the number average molecular weight (Mn), weight average molecular weight (Mw), z-average molecular weights (Mz) and Polydispersity (Mw/Mn) on both single and multi-component polymer ...
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Purity Analysis of Peptides or Proteins
A pure protein is one that is free from any quantifiable amounts of impurities. Any purity determination is only as reliable as the analytical methods used, and factors such as the structural properties of the protein itself, the amount of protein available, the nature of potential contaminants in the sample, and the accuracy of the estimate required should always be considered when selecting the method of ...
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Peptide Mass Fingerprinting (PMF) Services
Creative Proteomics provides Peptide Mass Fingerprinting (PMF) analysis and ions searching against database for rapid identification of ...
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Sequence Analysis of Peptides or Proteins (N—,C—)
Still N-terminal Edman sequencing has advantages for protein analysis that cannot readily be obtained by other analysis methods. Creative Proteomics provides N-terminal sequence analysis by both Edman and Mass spectrometry of therapeutic proteins, monoclonal antibodies and protein ...
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Membrane Protein Identification Services
Membrane proteins such as receptors and ion channels are key regulators of cellular function. Membrane proteins account for up to two thirds of known drugable target, highlighting their critical pharmaceutical ...
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Proteomics Service - Protein Quantification
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2D Fluorescence Difference Gel Electrophoresis (DIGE) Services
Creative Proteomics provides DIGE service which allows for simultaneous separation of up to three samples on one gel, bringing a new level of statistical confidence and reliability to 2D gel ...
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iTRAQ Protein Quantification Service
Creative Proteomics offers iTRAQ protein quantification service suited for unbiased untargeted biomarker discovery. Relative quantification of proteins for biomarker discovery in complex mixtures by mass spectrometry can easily and quickly be achieved using iTRAQ technology. iTRAQ is ideally suited for comparing normal, diseased, and drug-treated samples, time course studies, biological replicates and provides relative ...
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SILAC-based Proteomics Analysis
SILAC-based quantitative proteomics (SILAQ) is an innovative technology used in high throughput quantitative analysis of large protein complexes, protein-protein and protein-small molecule interactions. SILAC service of Creative Proteomics provides an unbiased strategy that can reveal how specifically either inhibitors, or other perturbations, affect the dynamic properties and cellular distributions of proteins. It can also be used as a sensitive and ...
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Absolute Quantification (AQIA) Services
Absolute Quantification is a targeted quantitative proteomics technique that exhibits robust efficacy and is being increasingly utilized for a wide variety of quantitative proteomics studies. AQUA strategy is for the absolute quantification (AQUA) of proteins and their modification states. Peptides are synthesized with incorporated stable isotopes as ideal internal standards to mimic native peptides formed by proteolysis. These synthetic peptides can also ...
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Label-free Quantification Services
Creative Proteomics provides Label-free methods for both relative and absolute quantification, which a rapid and low-cost alternative to other quantitative proteomic ...
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Semi-quantitative Proteomics Analysis Services
In scientific research, there are two main types of research analysis: qualitative and quantitative. Between these two extremes is semi-quantitative analysis, which assigns approximate measurements to data, rather than an exact measurement. Often used in cases where a direct measurement is not possible, but inference is unacceptable. In particular, the analysis is useful in cases where quantified data might fluctuate ...
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Protein Post-translational Modification Analysis
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Di-Sulfide Bond Localization Services
For many proteins and peptides, disulfide bridges are prerequisite for their proper biological function. Many commercialized proteins are cross-linked by disulfide bridges that increase their resistance to destructive effects of extreme environment used in industrial processes or protect protein-based therapeutics from rapid proteolytic degradation. Manufacturing of these products must take into account oxidative refolding—a formation of native ...
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Glycosylation Analysis of Protein
Glycosylation, the attachment of sugar moieties to proteins, is a post-translational modification (PTM) that provides greater proteomic diversity than other PTMs. Glycosylation is critical for a wide range of biological processes, including cell attachment to the extracellular matrix and protein-ligand interactions in the cell. This PTM is characterized by various glycosidic linkages, including N-, O- and C-linked glycosylation, glypiation (GPI anchor ...
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Protein phosphorylation Services
Protein phosphorylation is the most commonly studied area of post-translational modification since it plays a vital role in intracellular signal transduction and is involved in regulating cell cycle progression, differentiation, transformation, development, peptide hormone response, and adaptation. It has been estimated that one third of mammalian proteins may be phosphorylated and this modification often plays a key role in modulating protein function. ...
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Ubiquitination Services
Ubiquitin is an about 8.5 kDa polypeptide consisting of 76 amino acids that is appended to the ε-NH2 of lysine in target proteins via the C-terminal glycine of ubiquitin. Following an initial monoubiquitination event, the formation of a ubiquitin polymer may occur, and polyubiquitinated proteins are then recognized by the 26S proteasome that catalyzes the degradation of the ubiquitinated protein and the recycling of ...
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S-Nitrosylation Services
Nitric oxide (NO) is produced by three isoforms of nitric oxide synthase (NOS) and is a chemical messenger that reacts with free cysteine residues to form S-nitrothiols (SNOs). S-nitrosylation is a critical PTM used by cells to stabilize proteins, regulate gene expression and provide NO donors, and the generation, localization, activation and catabolism of SNOs are tightly ...
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Methylation Services
The transfer of one-carbon methyl groups to nitrogen or oxygen (N- and O-methylation, respectively) to amino acid side chains increases the hydrophobicity of the protein and can neutralize a negative amino acid charge when bound to carboxylic acids. Methylation is mediated by methyltransferases, and S-adenosyl methionine (SAM) is the primary methyl group donor. Methylation occurs so often that SAM has been suggested to be the most-used substrate in ...
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Proteomics Service - Top Down Proteomics
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Peptidomics Services
Top-down mass spectrometry, as an emerging approach for characterization of intact proteins, is established as a contrast to the bottom-up strategy, which is also called shotgun method, for analysis of peptides from enzymatic or chemical cleavage of intact proteins. Currently the bottom-up strategy is the most mature and most widely used approach for protein identification, characterization of post-translational modifications, and even relative and ...
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Top-Down based Sequencing Services
In top-down proteomics, intact protein molecular ions generated by ESI or MALDI are introduced into mass analyzer and are subjected to gas-phase fragmentation. Top-down analysis facilitates direct observation of C- and N-termini for identification of truncations and distinguish one isoform from the other by different ...
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Top Down-based Characterization Services of PTMs
Top-Down MS is powerful for comprehensive analysis of protein modifications because Top-Down MS is able to analyze intact proteins/peptides without proteolysis. Therefore, the strategy can preserve the labile modified motifs which are mostly lost when shotgun method is applied and CID is used for fragmentation in analysis of protein therapeutics, such as monoclonal antibodies and recombinant ...
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Characterization Services of Protein Structure
The application of Bottom-Up strategy is limited by incomplete or ambiguous characterization of alternative splice forms, diverse modifications and endogenous protein cleavages. As an alternative, the Top-Down strategy has been utilized more with the advancement of analytical instruments. Except for FT-ICR mass spectrometer, invention and innovation of orbitrap mass spectrometer has promote the development of top-down ...
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Metabolomics Service Overview
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Metabolomics Services
Metabolomics is the study of metabolism, specifically the science of identifying and quantifying the biochemical byproducts of metabolism, called cellular metabolites. This is achievedby using analytical technologies such as NMR and mass spectrometry combined with sophisticated statistical methods to interpret the generated data. Compared with genomics, transcriptomics and proteomics, metabolomics provide a direct and global snapshot of all the ...
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Untargeted Metabolomics Services
Untargeted metabolomics, also called discovery metabolomics, usually involves the comparison of the metabolome between the control and test groups, to identify differences between their metabolite profiles which may be relevant to specific biological ...
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Targeted Metabolomics Services
Targeted metabolomics refers to experiments that aim to accurately measure the abundance of previously identified metabolites, such as validation studies which typically include a large number of samples. It usually requires the use of analytical standards for absolute quantification. It may be used to confirm results from a previous discovery based metabolomic profiling experiment or based on results from a genomics and/or proteomics ...
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Lipidomics Services
Lipids are hydrophobic or amphipathic small molecules which include fats, waxes, sterols, fat-soluble vitamins (such as vitamins A, D, E and K), monoglycerides, diglycerides and phospholipids. The crucial role of lipids in biological physiology is evident not only in energy storage and structural components of cellular membranes, but also in signal transduction, membrane trafficking and ...
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Xenobiotic Metabolites Analysis Services
A xenobiotic is a foreign chemical substance found within an organism that is not normally naturally produced by or expected to be present within that ...
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Determination of Amino Acid Composition
Creative Proteomics can help you to determine the amino acid composition of various samples you submit. As the basic brick for peptides and proteins ,the amino acids are quite important because of the significance of protein/peptide physiological functions. Meanwhile the amino acid composition and levels are essential for nutrition and status of body health. For amino acid metabolomics, there are more than 300 kinds of amino acids and ~20 amino acids can ...
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Bioinformatics Services
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Gene Ontology (GO) Analysis Services
Gene ontology, GO for short, is a quite powerful bioinformatics initiative to unify the representation of gene and gene product attributes across all species. Gene Ontology is established by Gene Ontology Consortium in 2008 in order to annotate and classify genes and their corresponding products, mainly under 3 terms: molecular functions, cellular components and biological processes. More specifically, the project aims to: Maintain and develop its ...
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KEGG (Kyoto Encyclopedia of Genes and Genomes) Service
KEGG, abbreviation of Kyoto Encyclopedia of Genes and Genomes, is a collection of databases, which is used for bioinformatics research, including data mining in genomics, proteomics, metabolomics and other omics studies, modeling and simulation in systems biology, and translational research in drug R & ...
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Protein-Protein Interaction Networks Services
Proteins are vital macromolecules, the workhorses that facilitate most biological processes at both cellular and systemic levels, but they rarely act alone. Lots of essential molecular processes are carried out by molecular machines that are based on a large number of protein components organized by their Protein-Protein Interactions(PPIs), which refer to intentional physical contacts established between two or more proteins as a result of biochemical ...
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Cluster Analysis Services
In data mining, cluster analysis is used to classify a set of observations into two or more mutually exclusive unknown groups, based on combinations of the interval variables. The purpose, is to discover a system of organizing observations, usually genes, and proteins into groups, where members of the groups share properties in common. In Creative Proteomics, we can interpret the data you collected with a set of typical clustering methodologies, ...
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Technical - Analytical Service
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Gas Chromatography (GC) Based Analysis Services
Gas Chromatography (GC) is an analytical technique for a wide variety of volatile and/or semi-volatile organic compounds identification and quantitation. It is an indispensable technique for analytical lab to analysis a variety assortment sample types. GC facilitates the separation of complex sample matrices. Gas Chromatography (GC) Based Analysis Services at ...
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Liquid Chromatography (LC) Based Analysis Services
Liquid Chromatography (LC) is a form of chromatography that allows separation of complex mixtures of both semi- and non-volatile compounds based on slight differences in solubility between chemicals. This technique is a complementary to GC but effective when the compounds of interest are not volatile or are thermally labile. Liquid Chromatography (LC) Based Analysis Services ...
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High Performance Liquid Chromatography (HPLC) based Analysis Service
High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC utilized the quality and quantity of both small molecules and polymeric species.High Performance Liquid Chromatography (HPLC) Analysis Services at Creative-Proteomics offers you ...
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Ultra Performance Liquid Chromatography (UPLC) Based Analysis Service
Ultra Performance Liquid Chromatography (UPLC) is the newest technology in liquid chromatography based analysis. UPLC is the upgrade high performance liquid chromatography with high pressures, outstanding in both peak resolution and sensitivity. As well as higher sample throughput obtain more available sample information and the decrease separation time consumption. UPLC can provide us with impactful results within their organization. ...
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Gel Permeation Chromatography based Analysis Service
Gel Permeation Chromatography (GPC) at Creative-Proteomics supports your research in the MW and MW distribution measurement of polymers ranging from conventional soluble temperatures to high solvable temperatures. Gel Permeation Chromatography (GPC) based analysis Service at Creative-Proteomics offers you a state-of-the-art GPC platform to ...
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Gel Permeation Chromatography (GPC) & Size Exclusion Chromatography (SEC) based Analysis Service
Gel Permeation Chromatography (GPC) and Size Exclusion Chromatography (SEC) at Creative-Proteomics support your research in the molar mass and composition measure of polyolefins, characterize of aqueous and organic polymers matrices.Gel Permeation Chromatography (GPC) and Size Exclusion Chromatography (SEC) based analysis Service at Creative-Proteomics offers ...
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Ion Chromatography Based Analysis Services
Ion Chromatography at Creative-Proteomics supports your research in the identification and quantitation of both organic and inorganic ions ranging from conventional pharmaceutical industries to cosmetic industries. Ion Chromatography (IC) based analysis Service at Creative-Proteomics offers you a state-of-the-art IC platform to help you with ...
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High Performance Thin Layer Chromatography (HPTLC) based Analysis Service
High Performance Thin Layer Chromatography (HPTLC) at Creative-Proteomics supports your research in the qualitative and quantitative analysis, which is able to separate the compounds of the sample mixture. High Performance Thin Layer Chromatography (HPTLC) based analysis Service offers you a state-of-the-art High Performance Thin Layer Chromatography platform to help your research with all ...