Creative Proteomics services

Proteomics Service - Protein Identification

Sample Preparation Services

Sample preparation is critical for proteomics analysis, and Creative Proteomics provides sample preparation service according to your needs.

In-gel or In-solution Digestion Services

Among the endoproteases which could be used for protein digestion, the serine protease trypsin is most commonly employed as it generates peptides which are highly amenable to MS(/MS) analysis. Depending on the preceding workflow, the enzymatic digestion of proteins is performed either in-gel or in-solution, generally, the in-gel digestion methodology has become routine for proteins separated by 2-D electrophoresis while in-solution digestion are usually used in LC-MS/MS analysis. The protein is cut enzymatically into a limited number of shorter fragments during digestion and these fragments are called peptides and allow for the identification of the protein with their characteristic mass and pattern.

Molecular Weight Determination and Molecular Weight Distribution of Peptides or Proteins

Our comprehensive service and detailed reports will provide you with the number average molecular weight (Mn), weight average molecular weight (Mw), z-average molecular weights (Mz) and Polydispersity (Mw/Mn) on both single and multi-component polymer samples.

Purity Analysis of Peptides or Proteins

A pure protein is one that is free from any quantifiable amounts of impurities. Any purity determination is only as reliable as the analytical methods used, and factors such as the structural properties of the protein itself, the amount of protein available, the nature of potential contaminants in the sample, and the accuracy of the estimate required should always be considered when selecting the method of analysis.

Peptide Mass Fingerprinting (PMF) Services

Creative Proteomics provides Peptide Mass Fingerprinting (PMF) analysis and ions searching against database for rapid identification of proteins.

Sequence Analysis of Peptides or Proteins (N—，C—)

Still N-terminal Edman sequencing has advantages for protein analysis that cannot readily be obtained by other analysis methods. Creative Proteomics provides N-terminal sequence analysis by both Edman and Mass spectrometry of therapeutic proteins, monoclonal antibodies and protein vaccines.

Membrane Protein Identification Services

Membrane proteins such as receptors and ion channels are key regulators of cellular function. Membrane proteins account for up to two thirds of known drugable target, highlighting their critical pharmaceutical importance.

Proteomics Service - Protein Quantification

2D Fluorescence Difference Gel Electrophoresis (DIGE) Services

Creative Proteomics provides DIGE service which allows for simultaneous separation of up to three samples on one gel, bringing a new level of statistical confidence and reliability to 2D gel electrophoresis.

iTRAQ Protein Quantification Service

Creative Proteomics offers iTRAQ protein quantification service suited for unbiased untargeted biomarker discovery. Relative quantification of proteins for biomarker discovery in complex mixtures by mass spectrometry can easily and quickly be achieved using iTRAQ technology. iTRAQ is ideally suited for comparing normal, diseased, and drug-treated samples, time course studies, biological replicates and provides relative quantitation.