Cytena articles

ELISA plate washing is a critical determinant of assay performance. Residual liquid and carryover after washing can drive background signals, compressing the dynamic range and elevating the limit of detection.

Residual volume is the key variable. After aspiration-based washing, a small amount of liquid often remains in the well. That residual contains whatever you are trying to wash out. Even a few microliters can be enough to produce measurable signal when the detection enzyme is act