EpiCypher products

SNAP-ChIP® Spike-ins

SNAP-ChIP - K-MetStat Panel

A panel of distinctly modified mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431 or H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pairs of barcoded Widom 601 positioning sequence DNA. The panel is comprised of a pool of 1 unmodified plus 15 histone H3 or H4 post-translational modifications (PTMs, created by a proprietary semi-synthetic method): H3K4me1, H3K4me2, H3K4me3, H3K9me1, H3K9me2, H3K9me3, H3K27me1, H3K27me2, H3K27me3, H3K36me1, H3K36me2, H3K36me3, H4K20me1, H4K20me2 and H4K20me3. Each distinctly modified nucleosome is distinguishable by a unique sequence of DNA ("barcode") at the 3` end that can be deciphered by qPCR or next-generation sequencing. Each of the 16 nucleosomes in the pool is wrapped by 2 distinct DNA species, each containing a distinct barcode ("A" and "B", see SNAP-ChIP Manual) allowing for an internal technical replicate.

SNAP-ChIP - K-AcylStat Panel

A panel of distinctly modified mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431 or H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pairs of barcoded Widom 601 positioning sequence DNA. The panel is comprised of a pool of 1 unmodified plus 22 histone H2A, H3, or H4 post-translational modifications (PTMs, created by a proprietary semi-synthetic method): H3K4ac, H3K9ac, H3K14ac, H3K18ac, H3K23ac, H3K27ac, H3K36ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac, H4K20ac, H3K9bu, H3K9cr, H3K18bu, H3K18cr, H3K27bu, H3K27cr, H3K27acS28phos, H3K4,9,14,18ac, H4K5,8,12,16ac, H2AK5,8,13,15ac. Each distinctly modified nucleosome is distinguishable by a unique sequence of DNA (“barcode”) at the 3’ end that can be deciphered by qPCR or next-generation sequencing.

SNAP-CUTANA™ Spike-in Controls

SNAP-CUTANA - K-MetStat Panel

The SNAP-CUTANA K-MetStat Panel of spike-in controls for CUT&RUN and CUT&Tag offers an all-in-one solution to determine antibody specificity for histone posttranslational modifications (PTMs), monitor assay success, and normalize data for quantitative chromatin mapping. The panel contains designer nucleosomes (dNucs) representing 16 different K-methyl PTM states: mono-, di-, and trimethylation at H3K4, H3K9, H3K27, H3K36, & H4K20, as well as unmodified control (Figure 1). Each PTM is represented by two unique DNA-barcoded templates (A and B, for an internal technical replicate). Each dNuc is individually conjugated to paramagnetic beads and pooled into a single panel for convenient one-step spike-in to CUT&RUN and CUT&Tag experiments. The panel is added to samples alongside ConA-immobilized cells prior to the addition of an antibody targeting a histone lysine methylation state or IgG negative control (see Application Notes and Table 1).

CUTANA - pAG-MNase for ChIC/CUT&RUN Workflows

Available in 50 and 250 Reaction pack sizes. Recombinantly produced in E. coli, CUTANA pAG-MNase for ChIC/CUT&RUN Workflows is a fusion of Proteins A and G to Micrococcal Nuclease. This construct is useful in performing Chromatin Immunocleavage (ChIC) (1) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN) (2, 3). CUTANA pAG-MNase contains a C-terminal 6xHis epitope tag.

CUTANA - Rabbit IgG CUT&RUN Negative Control Antibody

This Rabbit IgG Antibody can be used to control for non-specific background signal in CUT&RUN and other applications. Pair with the H3K4me3 positive control antibody (EpiCypher 13-0041) for a well-controlled experiment.