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CUTANARabbit IgG CUT&RUN Negative Control Antibody

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This Rabbit IgG Antibody can be used to control for non-specific background signal in CUT&RUN and other applications. Pair with the H3K4me3 positive control antibody (EpiCypher 13-0041) for a well-controlled experiment.
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Immunogen: None
Formulation: Affinity-purified antibody in PBS pH 7.6
Storage and Stability: Stable for 1 year at 4°C from date of receipt
Prior to use, prepare a 1 mg/mL working stock dilution in CUT&RUN Antibody Buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 0.01% Digitonin, 2 mM EDTA). Store working stock at 4°C for 1 month. Use 0.5 µg of the working stock for each CUT&RUN assay. A typical yield for a CUT&RUN assay using 500,000 K562 cells is 2-5 ng DNA.