Identifica products

The Species Identification by Insertions/Deletions (SPInDel) kit allow the identification of human genetic material on a biological sample, a preliminary step in many investigations. The kit is an alternative to DNA sequencing that uses a conventional genotyping methodology similar to that employed with Short Tandem Repeats (STRs), involving multiplex PCR followed by fragment size determination using capillary electrophoresis. The identification of species with the SPInDel method is achieved by the amplification of six hypervariable regions in mitochondrial ribosomal RNA (rRNA) genes using highly conserved PCR primers. Each species is defined by a unique numeric profile of fragment lengths (i.e., a numeric barcode) resulting from the combination of the length of indel-rich regions.

The IDENTIFICA Transgenic Salmon Detection Kit detects the transgenic DNA construct used in the AquAdvantage salmon. In November 2015, the Food and Drug Administration (FDA), approved for commercialization the first transgenic animal, the AquAdvantage salmon, created by AquaBounty technologies. The AquAdvantage salmon is being approved for sale in several countries since 2016. This transgenic animal was designed to achieve market size about twice as fast as the native species, with accelerated growth being concentrated in its first year of life. This GM Atlantic salmon (Salmo salar) has a “all-fish” DNA construct containing the promoter and terminator regions of the antifreeze protein (AFP) gene from the ocean pout (Zoarces americanus) linked to the growth hormone coding region (cDNA) from the chinook salmon (Oncorhynchus tshawytscha).