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SPInDel Kit
The Species Identification by Insertions/Deletions (SPInDel) kit allow the identification of human genetic material on a biological sample, a preliminary step in many investigations. The kit is an alternative to DNA sequencing that uses a conventional genotyping methodology similar to that employed with Short Tandem Repeats (STRs), involving multiplex PCR followed by fragment size determination using capillary electrophoresis. The identification of species with the SPInDel method is achieved by the amplification of six hypervariable regions in mitochondrial ribosomal RNA (rRNA) genes using highly conserved PCR primers. Each species is defined by a unique numeric profile of fragment lengths (i.e., a numeric barcode) resulting from the combination of the length of indel-rich regions.
The kit identifies several mammalian species, including Cat (Felis catus), Dog (Canis familiaris), Cattle (Bos taurus), Goat (Capra hircus), Pig (Sus scrofa), Horse (Equus caballus), Rabbit (Oryctolagus cuniculus), Sheep (Ovis aries) and Mouse (Mus musculus). Several wildlife species are also identified (see complete list below).
The kit includes an allelic ladder to help in the analyses.
Components: SPInDel Primer Set (10x), SPInDel Allelic Ladder
Product Size: 50 reactions (K001AL), 100 reactions (K001BL)
Equipment: ABI Genetic Analyzer (310, 3100, 3130, 3500, 3730 or equivalent)
Dye: 6-FAM
Size Standard: GeneScan™ 500 LIZ®
Filter Set: G5
Separation Polymer: POP7, POP6, POP4
Sample Type: DNA
Sample Volume: 10 µL/reaction
Technique: Fragment size determination (similar to STRs)
Shipping Condition: Dry, room temperature
The identification of species with the SPInDel method is achieved by the amplification of six hypervariable regions in mitochondrial ribosomal RNA (rRNA) genes using highly conserved PCR primers. Each species is defined by a unique numeric profile of fragment lengths (i.e., a numeric barcode) resulting from the combination of the length of indel-rich regions.
The kit is an alternative to DNA sequencing that uses a conventional genotyping methodology similar to that employed with Short Tandem Repeats (STRs), involving multiplex PCR followed by fragment size determination using capillary electrophoresis. The kit includes a software to help in the data analyses.