IDT Biologika products

Single use stirred tank reactors (STRs) are another well-established incubation vessel for cell culturing in live virus manufacturing. At IDT Biologika, the STR technology can be used for both suspension culture and adherent culture. For adherent cell culture, Microcarrier Technology is utilized to provide a very large surface area for the attachment and growth of cells. Adherent cells require a large surface area, and this approach provides an environment for efficient virus harvest.

iCELLis systems were designed for adherent cell culture applications such as viral vaccine and gene therapy vector production, with the specific goal of simplification and scalability. 2D systems such as cell factories and roller bottles or microcarriers in stirred tank reactors can require a lot of preparation of equipment and manipulation. With iCELLis^® bioreactors, you have a fixed batch where cells attach, which is a porous material, and it creates a high surface area to optimize the yield. The cells grow in a 3D environment and there is excellent temperature, pH, and dissolved oxygen control. iCELLis^® technology is flexible from small scale to commercial volume, supporting easier process scale-up.

Wave Bioreactor systems are ready-to-process platforms for cell culture of biological products including live viruses. Culture medium and cells are aseptically transferred into a single-use, pre-sterilized bag, which is placed on an electric rocking base, inducing waves in the cell culture fluid to provide efficient mixing and oxygen transfer. This supports processes with volumes up to 200 L. We use a primary cell culture and not a permanent cell line. The virus seed is then aseptically transferred into the bag, permitting cell infection to occur. The infected culture is then incubated and subsequently harvested. The wave action limits shear stress on the cells in comparison to traditional stirred tanks. The bag is discarded after use, eliminating cleaning and sterilization to save time and mitigate cross-contamination. Wave bioreactors are another way to bring flexibility and speed to upstream processing.

Roller bottle systems are a simple incubation system used in cell culture for the manufacture of viral products. It is a 2D system that is used for the cultivation of adherent cells as it provides a very similar process to traditional culture flasks. We can manipulate a limited number of roller bottles with operators, however when a batch size increases to 800 bottles, to maintain sterility, IDT Biologika utilizes a robotic system. This approach supports an aseptic process and ensures it stays free of contamination.