Rapp OptoElectronic products

Microscopy and Imaging - Multi-Photon Microscopy

RAPP - Model MOM - Movable Objective Microscope for Two-Photon Imaging

The Movable Objective Microscope (MOM) by Sutter Instrument is a sophisticated two-photon microscope designed for advanced imaging within living specimens. This system features a unique design allowing three-dimensional movement and rotation of objectives while keeping the specimen stationary, optimizing the imaging process. The MOM integrates two independent microscopes: a wide-field section equipped with an Olympus vertical illuminator for standard epifluorescence, and a two-photon section that guides excitation laser light for deep tissue imaging. It supports precise and smooth X, Y, and Z movements with minimal drift and high reproducibility, akin to Sutter's MP-285 micromanipulator. This allows efficient Z-stack and mosaic image acquisition without moving the stage. The flexible horizontal light path facilitates conversion between upright and inverted configurations, suitable for imaging various non-horizontal surfaces. The MOM remains adaptable to evolving scanning technologies, supporting multiple scanner types, and offers customizable detector paths for optimal photon capture. Compatible with ScanImage software, it ensures seamless integration with imaging hardware.

RAPP - Model DF-Scope - Multi-Photon Imaging Package

The DF-Scope is a specialized multi-photon imaging package developed for the Olympus BX51WI upright microscope, designed specifically for laboratories engaged in electrophysiology and epifluorescent imaging. This package enhances the microscope's capabilities by integrating components essential for multi-photon imaging, leveraging a Ti:Sapphire laser for optimal performance. Key features include the incorporation of elements from the MOM system such as resonant and galvo scan boxes, controllers, detector paths, and high-sensitivity photomultiplier tubes (PMTs). These components facilitate the capture of light emitted from the sample's focal volume, a critical aspect of non-linear excitation imaging. The DF-Scope is equipped with dual detector paths, one positioned above and one below the sample, to maximize light collection. This configuration is especially beneficial for thin samples, such as brain slices, by utilizing sub-stage detectors for enhanced signal acquisition, adaptable with various Olympus condensers.